中文摘要：

目的：利用双向电泳技术分析大鼠肾小球蛋白质,以建立肾小球蛋白质组分析的方法。方法：选取体重约200g的雄性Wistar大鼠,分离肾小球并提取蛋白质。使用24cm、pH3-11的IPG胶条对蛋白质进行一相等电聚焦,采用12.5%聚丙烯酰胺凝胶进行二相分离。Decyder软件分析凝胶上的蛋白质点,切取1个蛋白质点,经过胰蛋白酶消化后,使用飞行质谱获取肽指纹谱,并用Mascot软件分析。结果：纯化的肾小球纯度在90%以上,在双向电泳的凝胶上,共发现1240个肾小球蛋白质;切取的蛋白质点经鉴定为CAPZA2蛋白质,Mascot得分为137分（阈值为61分）。结论：成功建立了双向电泳分析大鼠肾小球蛋白质组的方法。

英文摘要：

Objective：To establish the method of analyzing the proteomics of rat glomeruli by Two-dimensional gel electrophoresis （2-DE）.Methods：The glomeruli protein isolated from 200 g wistar rat was separated by 24 cm pH 3-11 IPG strip and 12.5% polyacrylamide gel.The number of protein spot was calculated by Decyder software.The protein spot was digested by tripsin and the peptide mapping fingerprint was obtained by Maldi-tof.The results were analyzed by Mascot software.Results：The purity of glomeruli was more than 90%.There were 1 240 spots being found in 2-DE map.The protein spot was identified as capping protein muscle Z-line alpha 2 （CAPZA2） protein with the value of 137 calculated by mascot （threshold was 61）.Conclusion：The method of analyzing the rat glomeruli by 2-DE map is established successfully.