中文摘要：

目的探讨体外阻断活化的供鼠T淋巴细胞CDl37-CDl37L共刺激途径抑制供鼠淋巴细胞的免疫反应。方法应用雄性供鼠BALB／c脾淋巴细胞为反应细胞，雌性受鼠C57BL／6脾淋巴细胞为刺激细胞，进行混合淋巴细胞培养（MLC）。设单抗实验组（加抗CDl37L单抗）和对照组（不加抗CDl37L单抗），初次和再次MLC第1、3、5、7天采用流式细胞术检测CD3＋CD4＋T细胞、CD3＋CD8＋T细胞；RT-PCR法检测培养的细胞IL-2、IFN-y、IL-4、IL-10的水平。结果实验组CD3＋CD8＋T细胞明显低于对照组（P〈O．01）；实验组IL-2、IFN-7水平明显低于对照组（P〈O．01）；实验组IL-10表达明显高于对照组（P〈O．01）。结论体外MLC中，应用抗CDl37L单抗孵育供鼠脾T淋巴细胞主要抑制供鼠CD3＋CD8＋T细胞的增殖，抑制I类细胞因子IFN-r及IL-2的表达，促进Ⅱ类细胞因子II：10的表达。

英文摘要：

Objective To investigate the immune efficiency of reliving graft-versus-host disease （GVHD） by in vitro blocking CD137-CD137L ligand costimulatory pathway in mice. Methods The spleen cells were taken from male BALB/c miceas the responder cells and those taken from female C57BL/6 as the stimulator cells, which underwent in vetro mixed lymph cell culture（MLC）. The cells were divided into two groups of A（adding anti-CD137L mAb） and B（without anti-CD137L mAb）. After treated with anti-CD137L mAb, primary and secondary MLC assay was used to detect the expression rates of CD3＋ CD8＋ T cells and CD3＊ CD4＋ T cells by flow cytometry, and the levels of cytokines（IFN-7, IL-2, IL-10 and IL-4） were determined by RT-PCtL Results The expression of CD3＋ CD8＋ T cells, but not CD3＋ CD4＋ T cells, was lower in group A than that in group B （P〈0. 01）. The expressions of IL-2 and IFN-7 were lower in group A than those in group B （P〈0. 01） and the expression of IL-10 was higher in group A than that in group B（P〈0. 01）. Conclusion In MLC system, anti-CD137L mAb can surpress the expression of CD3＋ CD8＋T cells, inhibit the production of cytokines I （IFN-）＇,IL-2） and accelerate the cytokines Ⅱ （IL-10） production.