中文摘要：

目的建立一种高效、稳定的从小鼠骨髓中分离培养与定向诱导分化内皮祖细胞的方法。方法通过密度梯度离心法从小鼠骨髓中分离单个核细胞,经差速贴壁结合特殊培养基扩增,诱导分化为内皮祖细胞。应用流式细胞技术鉴定内皮细胞系列标志：CD34、CD31、Flk-1和祖细胞标志CD133。结果经密度梯度离心和差速贴壁法分离所得的细胞经EBM-2专用培养基培养后,第4天可见集落形成,培养第12天流式细胞仪检测其CD34、CD133、Flk-1、CD31的阳性率分别为65%±4%、48%±3%、37%±3%和51%±4%。结论从小鼠骨髓中分离培养与定向诱导分化内皮祖细胞的方法效率高,稳定性和重复性好。

英文摘要：

Aim To establish an efficient and stable method for isolation,culture and directional differentiation of endothelial progenitor cells from mouse bone marrow. Methods Mononuclear cells were isolated from mouse bone marrow by density gradient centrifugation and differential adhesion method.The remaining cells were cultured and differentiated to endothelial progenitor cells in EBM-2.The expressions of specific antigens（CD34,CD133,Flk-1 and CD31） on cell surface were analyzed by flow cytometer. Results Cells were obtained from mouse bone marrow by density gradient centrifugation and differential adhesion method formed clusters at day 4.At day 12,positive ratios of CD34＋,CD133＋,Flk-1＋ and CD31＋ were 65%±4%,48%±3%,37%±3% and 51%±4%,respectively. Conclusion An efficient,stable and replicable method for isolation and culture of endothelial progenitor cells from mouse bone marrow has been established.