中文摘要：

目的：探讨电针对局灶性脑缺血大鼠微血管内皮细胞转运功能的影响机制。方法：60只Wistar雄性大鼠随机分为假手术组、模型组、电针组。采用线栓法制备局灶性脑缺血模型，电针组电针双侧“内关”穴（疏密波，4Hz／20Hz，强度1mA，持续30min）治疗15d。运用免疫组化法和蛋白免疫印迹法检测大鼠缺血侧海马微血管内皮细胞葡萄糖转运蛋白-1的表达与含量。结果：模型组大鼠缺血侧海马微血管内皮细胞葡萄糖转运蛋白-1的表达显著低于假手术组（P〈0．05），电针治疗后葡萄糖转运蛋白-1的表达与模型组比较显著升高（P〈0．05）。结论：电针可促进局灶性脑缺血大鼠海马微血管内皮细胞葡萄糖转运蛋白-1的表达，提高脑缺血后微血管内皮细胞的葡萄糖转运功能。

英文摘要：

Objective To observe the effect of electroacupuncture （EA） on the expression of glucose transporter-1 （GLUT-1） of hippocampal microvascular endothelial cells in focal cerebral ischemia （CI） rats. Methods Sixty male Wistar rats were randomly divided into sham-operation, model and electroacupuncture （EA） groups. CI model was established by middle cerebral artery occlusion with a piece of thread embolus. EA （4 Hz/20 Hz, 1 mA） was applied to bilateral ＂Neiguan＂（PC 6） for 30 min, once daily for 15 days. GLUT-1 expression in the hippocampus was detected with immunohistochemistry and Western blot, respectively. Results After the treatment, compared with the sham-operation group, the expression of GLUT-1 in microvascular endothelial cells of the hippocampus in model group shown by both histochemical and Western blot techniques decreased apparently （P〈0.05）. While in comparison with model group, the expression of GLUT-1 in EA group was upregulated significantly （P〈0.05）. Conclusion EA of PC 6 can effectively upregulate GLUT-1 expression in the hippocampus in cerebral ischemia rats, which may contribute to its effect in improving CI by raising the glucose transportation of microvascular endothelial cells.