中文摘要：

背景：骨髓间充质干细胞的成脂肪分化与成骨分化比例的失衡与许多骨疾病密切相关,而近些年发现Wnt信号通路在调控骨髓间充质干细胞的分化方向中起重要作用。目的：通过Wnt信号通路PCR基因芯片观察大鼠骨髓间充质干细胞分化为脂肪细胞和成骨细胞后相关基因表达的变化,寻找Wnt信号通路中调控骨髓间充质干细胞分化的靶基因。方法：采用大鼠第3代骨髓间充质干细胞分别进行成骨诱导和成脂诱导,7d后用Trizol萃取培养瓶中的细胞总RNA,倒置显微镜下观察骨髓间充质干细胞形态特征及成骨诱导后和成脂诱导后细胞形态特征。采用Wnt信号通路PCR芯片（大鼠）进行基因芯片检测,以未诱导组为对照,计算成脂肪诱导,成骨诱导后相关基因上调/下调的比值。结果与结论：①倒置显微镜下观察,传3代后可获得均一性较高的骨髓间充质干细胞,骨髓间充质干细胞经成骨诱导后向成骨细胞方向分化,经成脂诱导后向脂肪细胞方向分化。②与未诱导组相比,骨髓间充质干细胞成脂诱导后Wnt信号通路表达上调的基因（Dkk-1,kremen,FZD1,FZD7）等15个（ratio〉2）,下调的基因（sFrp5,β-catenin,Dvl3,Tcf7）等16个（ratio〈0.5）。成骨诱导后,Wnt信号通路表达上调的基因（Dkk1,kremen,β-catenin,Wnt11）6个,表达下调的基因（sFrp5,sFRP4,Fzd1）等15个。提示Wnt信号通路在骨髓间充质干细胞成脂细胞分化和成骨细胞分化中发挥重要作用。

英文摘要：

BACKGROUND： Disequilibrium of proportion of adipogenesis and osteogenesis from bone marrow mesenchymal stem cells （BMSCs） is associated with many bone diseases. However, it has been demonstrated that Wnt signaling pathway could play an important role in regulation of BMSC differentiation. OBJECTIVE： To investigate the different gene expression profiles and to find the target gene on Wnt signaling pathway of the BMSCs, after being induced to osteoblasts and adipocytes respectively using Wnt signaling pathway PCR array. METHODS： The third-passage BMSCs, after being induced to osteoblasts and adipocytes respectively for 7 days. The total mRNA of MSCs was extracted by Trizol. BMSC morphology was observed following osteogenic and adipogenic induction under an inverted microscope. Gene array was detected by rat Wnt signaling pathway PCR array. Non-induction group served as controls. The ratio of increase/reduction gene of osteoblasts and adipocytes was calculated. RESULTS AND CONCLUSION： Under an inverted microscope, BMSCs with high homogenicity were obtained following passage3. BMSCs differentiated into osteoblasts following osteogenic induction, and into adipocytes following adipogenic induction. Compared with non-induction group, fifteen genes （Dkk1, kremen, FZD1, FZD7, et al.） were expressed up-regulated （ratio 2） and 16 （sFrp 5, β-catenin, Dvl3, Tcf7, et al.） genes down-regulated （ratio 0.5） when the third-passage BMSCs were induced toadipocytes. Six genes （Dkk1, kremen, β-catenin, Wnt11, et al.） were expressed up-regulated and 15 genes （sFrp5, sFRP4, Fzd1,et al.） down-regulated when BMSCs being induced to osteoblasts. Above-mentioned results suggested that Wnt signaling pathway plays an important role in the osteoblast and adipocyte differentiation from BMSCs.