中文摘要：

目的 克隆人疱疹病毒6型（HHV-6）特异性CD4^＋ T细胞,并分析其基本特征。方法采用微孔有限稀释法克隆HHV-6特异性CD4^＋ T细胞;3H-TdR掺入法细胞增殖试验筛选HHV-6特异性CD4^＋ T细胞克隆;流式细胞仪（FACS）分析HHV-6特异性CD4^＋ T细胞克隆的表面标志,酶联免疫吸附试验（ELISA）检测HHV-6特异性CD4^＋ T细胞克隆的细胞因子分泌水平。结果 获得的细胞克隆中有4株以HHV-6感染细胞裂解物特异的方式增殖,并且其增殖水平与HHV-6感染细胞裂解物呈剂量依赖性;细胞克隆表面标志为CD3＋CD4^＋;W-2和W-4细胞克隆的细胞因子分泌以白细胞介素10（IL-10）为主,W-1细胞克隆的细胞因子分泌以IL-10及γ干扰素（IFN-γ）为主,W-3细胞克隆的细胞因子分泌以IFN-γ为主。结论 初步建立HHV-6特异性CD4^＋ T细胞克隆,并分析其表面标志和细胞因子分泌水平,为HHV-6特异性Treg细胞的克隆、筛选及其功能的研究奠定基础。

英文摘要：

Objective To establish HHV-6-specific CD4^＋ T cell clones and to study the characteristics of HHV-6-spocific CD4^＋ T cells. Methods We obtained CD4^＋ T cell clones by using the liquid microwell limiting dilution technique, and selected HHV-6-spocific CD4^＋ T cell clones by means of ^3H-thymidine uptake. The phenotype of the HHV-6-specific T cell clones was analyzed with FACS. We determined IL-10,IL-4 and IFN-γ secretion from HHV-6-specific CD4^＋ T cell clones by ELISA.Results Of the five HHV-6-spocific CD4^＋ T cell clones, four showed proliferative responses to stimulation with HHV-6-infected JJHAN lysates. Moreover, proliferation responses of HHV-6- specific CD4^＋ T cell clones depended upon the concentration of the HHV-6-infected JJHAN lysates. The phenotypic analysis with FACS demonstrated that HHV-6-specific T cell clones consisted of CD3^＋ CD4^＋ T cells. The CD4^＋ T cell clones W-2 and W-4 had Trl-type profiles while the CD4^＋ T cell clone W-1 had a Th0-type profile and the clone W-3 had a Thl-type profile. Conclusion In the current study,we established HHV-6-specific CD4^＋ T cell clones in order to examine their particular phenotype, the nature of cytokine secretion, and to provide an experimental basis for selecting HHV-6-specific CD4^＋ Treg cell clones.