中文摘要：

构建了大豆幼胚生长期20～50dDSN（duplex-specific nulease）均一化cDNA酵母双杂文库，总克隆数为2．8×10^7cfu。为了筛选能与已知大豆bHLH转录因子GmbHLH3a相互结合的蛋白质，以转录因子GmbHLH3a为诱饵蛋白构建诱饵载体，GmbHLH3a诱饵载体无自激活活性，通过酵母双杂筛选到了两个能与诱饵蛋白相互作用的蛋白及其序列。

英文摘要：

In present study, DSN （duplex-specific nulease）-normalization yeast two-hybrid cDNA libraries of soybean seed development from 20 to 50 days after flowering were constructed. The total clone number was 2. 8 ×10^7 cfu. In order to obtain the proteins that could bind with the known soybean bHLH transcription factor GmbHLH3a,the bait plasmid of GmbHLH3a, whose carrier has no auto-activation, was constructed. Through yeast two-hybrid screening, two proteins which could interact with the bait plasmid and their corresponding sequences were acquired.