中文摘要：

目的研究铅对五指山猪（WZSP）肝脏的毒性作用，为研究铅对人类肝脏损伤的风险评估提供理论参考。方法将20头健康3月龄清洁级WZSP随机分为5组，每组4头（雌雄各半，雄性去势），用含铅量分别为0．046、6．790、17．370、53．600和154．000mg／ks的饲料对其进行饲喂染毒，连续100d。观察肝脏表观特征的变化，并测定血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）和γ-谷氨酰基转移酶（γ-GGT）的活力。结果随着染铅剂量的增高，ALT活力呈现明显的上升趋势，差异均有统计学意义（P〈0．05或P〈0．01）；AST活力也呈现递增的趋势，但与对照组相比，差异无统计学意义（P〉0．05）；γ-GGT活力逐渐升高，154．000mg／kg染铅组γ-GGT活力高于对照组，差异有统计学意义（P〈0．05）；ALP活力与对照组相比，差异均无统计学意义（P〉0．05）。154．000mg／ks染铅组的肝脏表观特征发生变化，出现颜色变暗，包膜与实体脱离及黄疸症状。结论铅暴露可对WZSP肝脏产生毒性作用。

英文摘要：

Objective To study the adverse effects of lead on the livers in minipigs,which can provide theoretical basis for risk assessment on human hepatic trauma. Methods Twenty pigs （Wuzhishan pig in Hainan of China,WZSP）aged 3 months, clean grade, were randomly divided into five groups, and they were fed with the feeds in which the lead contents were 0.046 mg/kg, 6.790 mg/kg,17.370 mg/kg,53.600 mg/kg and 154.000 mg/kg respectively for 100 days. The changes of livers apparent characteristics were observed and the activities of glutamic-pyruvic transaminase （ALT）,aspartate transcarbamoylase （AST）, alkaline phosphatases （ALP） and gamma glutamy transferase （γ-GGT） in the serum were determined. Results As the increase of lead dose,the activity of ALT in the serum increased significantly （P〈0.05 ,P〈0.01 ）;the activity of AST also increased gradually, but compared with the control group, there was no significant difference（P〉0.05）;the activity of γ-GGT increased gradually, in the group of 154.000 mg/kg,the activity ofγ-GGT was significantly higher than that in the control group （P〈0.05） ;compared with the control group, there were no significant differences among the activities of ALP in various lead doses （P〉0.05）. Compared with the other dose groups, in the 154.000 mg/kg group, the livers apparent characteristics changed, the colour of the liver became darker, hepatic envelope separated and jaundice appeared. Conclusion Lead exposure can cause hepatic damage in WZSP.