中文摘要：

细胞捕获分离是免疫学、诊断检测、病理研究等学科经常用到的生物学实验方法.近年来,微流控芯片平台的细胞捕获分离方式花样繁多,层出不穷,它具有可快速检测、所需样本量少、节约试剂、成本低廉等优势.本文主要对近年来多种微流控细胞捕获分离的方法,以免疫捕获分离和无标签细胞分离两类对其进行介绍.免疫捕获分离是较为传统的细胞捕获分离方式,它的特异性好、捕获分离后的细胞纯度较高.无标签细胞分离是近几年热门发展的技术手段,它采用物理学与生物学相结合的方式,能较好地保持细胞的完整性和生物活性.细胞捕获分离在微流控平台的应用虽然发展迅速,但其在工业化生产和微型化整合等方面还存在一些问题,只有解决生产问题,细胞捕获分离在微流控平台的应用才真正具有实际价值,可以真正作为一种技术手段用于日常的实验操作和医学检测中.就目前而言,细胞捕获分离在微流控芯片中仍具有很大的发展前景.

英文摘要：

Cell capturing and separation is frequently involved in researches in immunology, diagnostics and pathology.In recent years, different new methods for cell capturing and separation based on microfluidic platform have been developed.With microfluidic chip, rapid and low cost detection can be implemented with small sample and reagent volumes.In this review, two methods of microfluidic cell separation, immune-capture and label-free separation, are introduced.Immuno-capture, a more traditional method, can separate cells with high specificity and provide highly purified target cells.Newly developed label-free method performs separation by utilizing the physical and biological features of target cells, and can keep cell＇s integrity and activity.Although there are a couple of issues to be resolved before microfluidic cell separation gets widespread commercial use, such as multi-system integration and industrial-scale manufacture, it has already attracted extraordinary research attention and has been in the path on rapid development.It is believed that cell capturing and separation methods based on microfluidic chip have broad application prospect in life science and biomedical field.