中文摘要：

一个灵巧的表面电浆子回声(SPR ) 薄片为小分子决心和分析被开发。SPR 薄片基于装配原则，修改牛的浆液白朊(BSA ) 在是直接自我装配的到赤裸的金表面上的自我被准备。有修改 BSA 的薄片的表面形态学被原子力量显微镜学(AFM ) 调查，它的光性质被描绘。有一致形态学的赤裸的灵巧的 SPR 芯片的表面绑定能力是 8 次赤裸的控制 SPR 芯片的。基于在皮质醇抗体和皮质醇衍生物之间的有免疫力的反应的实验，有修改 BSA 的灵巧的 SPR 薄片的敏感比有未修改的 BSA 的控制 SPR 薄片的高得多。灵巧的 SPR 薄片成功地被用来检测小分子。最低察觉限制是有为皮质醇分析的 5100 ng/mL 的一个线性范围的 5 ng/mL。新奇灵巧的 SPR 薄片能也被使用检测另外的小分子。

英文摘要：

A facile surface plasmon resonance（SPR） chip is developed for small molecule determination and analysis. The SPR chip was prepared based on a self assembling principle, in which the modified bovine serum albumin（BSA） was directly self-assembled onto the bare gold surface. The surface morphology of the chip with the modified BSA was investigated by atomic force microscopy（AFM） and its optical properties were characterized. The surface binding capacity of the bare facile SPR chip with a uniform morphology is 8 times of that of the bare control SPR chip. Based on the experiments of immune reaction between cortisol antibody and cortisol derivative, the sensitivity of the facile SPR chip with the modified BSA is much higher than that of the control SPR chip with the un-modified BSA. The facile SPR chip has been successfully used to detect small molecules. The lowest detection limit is 5 ng/m L with a linear range of 5-100 ng/m L for cortisol analysis. The novel facile SPR chip can also be applied to detect other small molecules.