中文摘要：

背景神经干细胞(NSC ) 移植和基因治疗广泛地为然而，对待 cerebullar 和 myelonic 损害被调查了眼科学上的研究是稀罕的。这研究的目的是调查导出大脑的神经营养的因素(BDNF ) 的迁居和区别转基因的 NSC 移植了进正常老鼠视网膜的基因。方法 NSC 分别地在 vitro 有教养、净化、与 recombinant retrovirus pLXSN-BDNF 和 pLXSN 感染，到获得 BDNF overexpressed NSC (BDNF-NSCs ) 并且控制房间(p-NSCs ) 。在二转基因的 NSC 和未经治疗的 NSC 的 BDNF 基因的表示被测量由荧光灯量的聚合酶链反应(FQ-PCR ) 和连接酶的 immunosorbent 试金(ELISA ) 。BDNF-NSCs 和 NSC 感染联系 adeno 的提高病毒的绿色荧光灯蛋白质(AAV-EGFP ) 到在 vivo 追踪他们并且为移植用作施主房间直到正常老鼠视网膜的 subretinal 空格， phosphated 缓冲区答案(PBS ) 为否定控制用作假移植。在主人视网膜的施主房间的幸存，移植，和区别分别地与海德堡视网膜 angiograph (HRA ) 和 immunohistochemistry 被观察并且分析。结果 NSC 被限制冲淡试金成功地净化。在 BDNF-NSCs 的 BDNF 基因的表示在 FQ-PCR 测试的 mRNA 水平两个都在三个组之中是最高的(P < 0.05 ) 并且在 ELISA 测量的蛋白质水平(P < 0.05 ) ，它证明 BDNF 是在 BDNF-NSCs 的 overexpressed。HRA 的结果证明接枝房间能幸存很好并且移居进主人视网膜，当 immunohistochemical 分析揭示了与控制 NSC 相比更高效地区分进神经原的那移植 BDNF-NSCs 时在移植以后的 2 个月。结论 NSC 的种子房间高度， secreting BDNF 被建立。BDNF 能支持 NSC 在正常宿主视网膜移居并且区分进神经细胞。

英文摘要：

Background Neural stem cells （NSCs） transplantation and gene therapy have been widely investigated for treating the cerebullar and myelonic injuries, however, studies on the ophthalmology are rare. The aim of this study was to investigate the migration and differentJatJon of brain-derived neurotrophic factor （BDNF） gene transgenic NSCs transplanted into the normal rat retinas. Methods NSCs were cultured and purified in vitro and infected with recombinant retrovirus pLXSN-BDNF and pLXSN respectively, to obtain the BDNF overexpressed NSCs （BDNF-NSCs） and control cells （p-NSCs）. The expression of BDNF genes in two transgenic NSCs and untreated NSCs were measured by fluorescent quantitative polymerase chain reaction （FQ-PCR） and enzyme-linked Jmmunosorbent assay （ELISA）. BDNF-NSCs and NSCs were infected with adeno-associated viruses-enhanced green fluorescent protein （AAV-EGFP） to track them in vivo and served as donor cells for transplantation into the subretinal space of normal rat retinas, phosphated buffer solution （PBS） served as pseudo transplantation for a negative control. Survival, migration, and differentiation of donor cells in host retinas were observed and analyzed with Heidelberg retina angiograph （HRA） and immunohistochemistry, respectively. Results NSCs were purified successfully by limiting dilution assay. The expression of BDNF gene in BDNF-NSCs was the highest among three groups both at mRNA level tested by FQ-PCR （P 〈0.05） and at protein level measured by ELISA （P 〈0.05）, which showed that BDNF was overexpressed in BDNF-NSCs. The results of HRA demonstrated that graft cells could survive well and migrate into the host retinas, while the immunohistochemical analysis revealed that transplanted BDNF-NSCs differentiated into neuron more efficiently compared with the control NSCs 2 months after transplantation. Conclusions The seed cells of NSCs highly secreting BDNF were established. BDNF can promote NSCs to migrate and differentiate into neural cells in