本文提出了以十六烷基三甲基溴化铵修饰碳糊电极(CTAB/CPE)直接测定尿酸(UA)的新方法,用线性扫描伏安法(LSV)研究尿酸的电化学行为。在0.1mol/L磷酸盐缓冲溶液(PBS,pH=7.4)中,尿酸在CTAB/CPE上于0.160V处产生一个灵敏的氧化峰,氧化峰电流Ip与尿酸浓度(CuA)在1.0×10^-5—1.0×10^-3mol/L范围内呈良好的线性关系,相关系数为0.9940,检出限为1.0×10^-6mol/L。该方法简单实用,可以直接用于人体尿液中尿酸的检测。
In this work, a cetyltrimethylammonium bromide (CTAB) modified carbon paste electrode (CTAB/CPE) was developed on the basis of a surface modification method, which displayed an excellent electrochemical activity toward the oxidation of uric acid (UA). The electrochemical behaviors of UA at CTAB/CPE were investigated by linear sweep voltammetry(LSV). A sensitive oxidation peak of UA was observed at 0. 160 V for CTAB/CPE in 0.1 mol/L PBS (pH=7.4). The oxidation peak current had a good linear relationship with the concentration of UA in the range of 1.0×10^-5-1.0×10^-3mol/L, with a low detection limit of 1.0×10^-6mol/L. This modified electrode was applied to the determination of UA in human urine samples and the result was satisfied.