中文摘要：

目的 研究胰岛素诱导基因2（INSIG2）rs10185316多态性与儿童青少年血压指标的关联。方法 2005和2007年分别在北京市东城区9所初中和海淀区5所中小学（3所初中和2所小学），按照中国学龄儿童青少年超重、肥胖筛查BMI分类标准，招募肥胖超重学生1 425例，正常体重学生605名，选取其中血压和基因分型数据完整的2 018名研究对象，依据儿童青少年血压偏高标准，分为血压偏高组（共702例）和非血压偏高组（共1 316名）。通过问卷调查和体格检查收集研究对象的性别、年龄和体格指标（身高、体重和血压等），采集外周血样本并提取基因组DNA，对INSIG2 rs10185316多态性进行分型。采用多重线性回归模型分析INSIG2 rs10185316多态性与SBP、DBP、平均动脉压（mean arterial blood pressure, MAP）及脉压差的关联。结果 血压偏高组的年龄、BMI、SBP和DBP分别为（14.3±1.4）岁、（27.3±4.2）kg/m2、（130.5±10.9）和（76.7±13.3）mmHg（1 mmHg=0.133 kPa），均高于非血压偏高组[分别为（12.2±2.9）岁、（22.0±4.0）kg/m2、（104.4±10.9）和（54.6±15.2）mmHg]（P值均〈0.001）。调整年龄、性别、城区和BMI后，与INSIG2 rs10185316位点CC基因携带者相比，CG/GG基因型携带者DBP较低[β（95%CI）：-1.67（-2.84~-0.50），P=0.005]，脉压差较高[β（95%CI）：1.91（0.61~3.20），P=0.004]，MAP较低[β（95%CI）：-1.03（-2.01~-0.05），P=0.039]。结论 INSIG2 rs10185316多态性与儿童青少年DBP、脉压差和MAP相关，其作用独立于BMI。

英文摘要：

Objective To examine the association between polymorphism of rs10185316 in insulin-induced gene 2 （INSIG2） and blood pressure among children and adolescents.Methods 9 junior middle schools in Dongcheng District of Beijing and 5 schools （3 primary junior middle schools, 2 primary schools） in Haidian District of Beijing were chosen in 2005 and 2007, respectively. According to the Chinese BMI percentile criteria for screening overweight and obesity in school children, we recruited 1 425 overweight or obese children and 605 normal weight children. A total of 2 018 students with complete data of blood pressure and genotype data were included in this study. According to the blood pressure criterion of children and adolescents, 702 participants were categorized into high blood pressure group and 1 316 into normal blood pressure group. Participants＇ information of gender, age, height, weight and blood pressure were collected by questionnaire and physical examination. Genomic DNA was extracted from peripheral blood sample for genotyping of INSIG2 rs10185316 polymorphism. Multiple linear regression was conducted to analyze the associations between rs10185316 polymorphism in INSIG2 and SBP, DBP, mean arterial pressure （MAP） and pulse pressure.Results The age, BMI, SBP and DBP of the high blood pressure group were separately （14.3±1.4） years old, （27.3±4.2） kg/m2, （130.5±10.9） and （76.7±13.3） mmHg （1 mmHg=0.133 kPa）, all higher than that of the normal blood pressure group, which were （12.2±2.9） years old, （22.0±4.0） kg/m2, （104.4±10.9） and（54.6±15.2） mmHg, respectively （all P values〈0.001）. After age, sex, district and BMI adjusted, compared with the participants carrying INSIG2 rs10185316 CC genotype, CG/GG genotype carriers had lower DBP （β（95%CI）：-1.67（-2.84--0.50）, P=0.005）, higher PP（β（95%CI）： 1.91（0.61-3.20）, P=0.004）, and lower MAP（β（95%CI）：=-1.03（-2.01--0.05）, P=0.039）.Conclusion INSIG2 rs10185316 polymorp