中文摘要：

目的研究新型免疫抑制剂芬戈莫德对增生性瘢痕的抑制作用。方法 40只新西兰兔(雌雄各半)于兔耳腹侧面瘢痕造模,每只兔每侧耳造模创面2个。按照完全随机的分组原则将所有兔分为5组,每组8只,每组32个瘢痕创面,按照溶剂、不同浓度芬戈莫德分别注射的处理方式分为:空白对照组、溶剂对照组、芬戈莫德2.5 mg/m L组、芬戈莫德5.0 mg/m L组、芬戈莫德10.0 mg/m L组。瘢痕造模成功后一次性给药,观察并记录各组创面瘢痕大小变化,给药后第5天取材,对瘢痕组织进行组织学染色,量化并比较两组瘢痕创面的瘢痕表皮厚度、瘢痕胶原蛋白沉积情况及转化生长因子-β、结缔组织生长因子的蛋白表达情况。结果芬戈莫德各浓度组兔耳余量瘢痕面积比明显缩小;芬戈莫德2.5 mg/m L组瘢痕表皮厚度为(120.250±6.042)μm,瘢痕表皮增厚明显,胶原沉积率降低,分别与芬戈莫德5.0 mg/m L组、芬戈莫德10.0 mg/m L组比较,芬戈莫德2.5 mg/m L组与瘢痕增生相关的转化生长因子-β、结缔组织生长因子含量下降,差异均有统计学意义(P<0.05)。结论新型免疫抑制剂芬戈莫德对瘢痕形成增生有抑制作用,芬戈莫德浓度为2.5 mg/m L时效果最为显著。

英文摘要：

Objective To determine the therapeutic effect of fingolimod on hypertrophic scars. Methods Forty New zealand rabbits were used to construct hypertrophic scar models on the ears,and two masses of hypertrophic scars on each ear.The rabbits were divided into 5 groups by handling method:negative control group,solvent control group,fingolimod 2.5 mg/mL group,fingolimod 5.0 mg/mL group, fingolimod 1 0.0 mg/mL group,each group had 8 rabbits and 32 scare wound.The thickness and mass of hypertrophic scars were measured respectively,on the 5th day after injecting drug,the hypertrophic scars were harvested and detected by histological staining.The scar cuticle thickness,scar collagen deposition and protein expression of transforming growth factor beta and connective tissue growth factor were compared. Results The mass of hypertrophic scars in group fingolimod decreased significantly,the thickness of fingolimod 2.5 mg/mL group was (1 20.250 ±6.042)μm,collage deposition depressed.Compared to fingolimod 5.0 mg/mL,1 0.0 mg/mL group,the expression of transforming growth factor-βand connective tissue growth factor of fingolimod 2.5 mg/mL group descended,the difference was statistically significant (P <0.05).Conclusion Fingolimod can inhibit the hypertrophic scars,and 2.5 mg/mL fingolimod has the most significant effect.