中文摘要：

采用点滴法,于室内测定了阿维菌素、茚虫威和鱼藤酮3种杀虫剂单独使用及与绿僵菌混用对沙葱萤叶甲3龄幼虫的协同致死作用,以期为协调使用化学药剂和生防制剂防治沙葱萤叶甲提供参考。3种杀虫剂单独使用时的毒力测定结果表明,阿维菌素对沙葱萤叶甲3龄幼虫的毒力最强,LD50值为4.80 ng/头,其次是茚虫威和鱼藤酮,LD50值分别为10.47和53.21 ng/头。采用不同浓度的3种杀虫剂分别处理绿僵菌24和48 h后,发现药剂对绿僵菌分生孢子萌发均有一定的抑制作用,但抑制率低于20%,且抑制率随着药剂浓度的降低和作用时间的延长而降低,其中茚虫威的平均抑制率最小,分别仅为1.89%±0.57%和0.89%±0.39%。将3种杀虫剂分别与绿僵菌孢子悬液混配使用,对防治沙葱萤叶甲3龄幼虫均具有协同增效作用：其中茚虫威与绿僵菌混用增效作用最强,比单独施用绿僵菌时LT50值缩短了8.12 d;阿维菌素与绿僵菌混用时LT50值缩短了7.60 d;鱼藤酮与绿僵菌混用时LT50值缩短了6.45 d10。

英文摘要：

In order to provide reference for the coordination of chemical pesticides and biological control agents against Galeruca daurica Joannis, the joint lethal effect of three insecticides （ abamectin, indoxacarb and rotenone） mixed with Metarhizium anisopliae var. acridum on the third instar larvae of G. daurica was measured by topical application method. The results showed that the toxicity of abamectin to the larvae was the highest among the three insecticides tested, with LDs0 value of 4.80 ng/larva; the toxicity of indoxacarb and rotenone was lower than that of abamectin, with LDs0 value of 10.47 ng/larva and 53.21 ng/larva, respectively. Meanwhile, the inhibition of different concentration of insecticides on the germination of conidia of M. anisopliaewas determined after treatment for 24 h and 48 h. All the three pesticides showed inhibition on the spore germination of M. anisopliae, but the inhibition rates were less than 20%, and the inhibition decreased as the concentration decreased and the time prolonged. Indoxacarb showed the least influence on M. anisopliae after 24 h and 48 h, and the average inhibition rates were 1.89% ±0.57% and 0.89%±0.39%, respectively. The mixture of spore suspension of M. anisopliae and three pesticides against the third instar larvae of G. daurica was more effective than that of M. anisopliae alone, displaying clear synergetic activity, and the synergism of indoxacarb and M. anisopliae was the best. The LTs0 values of mixtures of M. anisoplia ＋ indoxacarb, M. anisopliae ＋ abamectin and M. anisopliae ＋ rotenone against G. daurica decreased by about 8.12 d, 7.60 d and 6.45 d, respectively, comparing with those of M. anisopliae alone.