中文摘要：

根据GenBank上发表的猪附红细胞体全基因组序列（登录号：NC_015153．1），针对其中的信号识别颗粒（SRP）基因设计合成一对特异性引物，应用PCR方法扩增猪附红细胞体信号识别颗粒54（SRP54）基因片段，克隆至pMD18-Tsimple载体上，经PCR、酶切初步鉴定正确后进行测序，对所克隆片段进行生物信息学分析，并构建重组表达质粒pGEX-SRP54。结果显示，克隆的SRP54基因片段大小为1164bp，与GenBank中参考序列的同源性为97％；该基因编码381个氨基酸，编码蛋白的等电点为6．51，无跨膜区；重组表达质粒pGEX-SRP54构建正确。

英文摘要：

According to the complete genome sequence of Eperythrozoon suis published in GenBank（NC_ 015153.1）,a pair of specific primers were designed and synthetized against the signal recognition particle gene sequence. The signal recognition particle 54 gene fragment of Eperythrozoon suis was amplified by PCR and cloned to pMD18-T simple. After preliminary identification correctly by PCR and enzyme digestion,sequencing was conducted. Then bioinformatics analyseis was conducted, and the recombinant expression plasmid pGEX-SRP54 was constructed. The results showed that the gene fragment Of SRP54 was 1 164 bp, and 97% homology with corresponding sequences in GenBank; It encoded 381 amino acids and the isoelectric point was 6.51. There was no transmembrane area; The recombinant expression plasmid of pGEX-SRP54 was constructed correctly, which laid the foundation for the follow-up studies.