中文摘要：

1 糖尿病( F1D )是的背景暴发性的类型复杂 disease.Microarray 分析被用来识别基因表示变化并且获得内在的 mechanisms.Methods Microarray 理解分析从六个 F1D 病人和六匹配的健康subjects.Real时间在外部血 mononuclear 房间上被执行聚合酶链反应被用来证实差别表示了 genes.NK 房间活动被识别的甲基 thiazoleterazolium assay.Results Microarray 分析检测

英文摘要：

Background Fulminant type 1 diabetes （F1D） is a complex disease. Microarray analysis was used to identify gene expression changes and obtain understanding of the underlying mechanisms. Methods Microarray analysis was performed on peripheral blood mononuclear cells from six F1D patients and six matched healthy subjects. Real-time polymerase chain reaction was used to verify the differentially expressed genes. NK cell activity was detected by methyl thiazoleterazolium assay. Results Microarray analysis identified 759 genes differing in expression between F1D patients and controls at a false discovery rate of 0.05. Expression of TLR9, ELF4 and ILIRAP were verified and consistent with changes in microarray results. NK cell activity was decreased in FID. With use of a knowledge base, differentially expressed genes could be placed within different pathways with predicted functions including interleukin-1, and tumor necrosis factor-a signaling. Conclusions These results identify several genes indicating possible mechanisms in FID. NK cell dysfunction resulting from changes in expression of TLR9, ELF4 and IL1RAP, and pathways of interleukin-1 and tumor necrosis factor-a sianalino miaht be involved in F1D throuoh inducino B-cell dysfunction.