中文摘要：

目的：通过对影响对叶百部碱纯化因素的研究及优化，得到对叶百部碱的最佳纯化工艺。方法：在建立HPLC—ELSD法测定对叶百部碱含量的基础上，采用单因素实验考察不同pH值、萃取溶剂、萃取次数和结晶溶剂对叶百部碱分离纯化的影响，筛选最佳纯化条件。结果：对叶百部药材醇提物酸化离心后，取上清液碱化至pH8左右，以萃取溶剂为石油醚-乙酸乙酯（1：1）为萃取溶剂萃取1次得到总碱浸膏。总碱溶于甲醇中结晶得到纯度大于98％的柱状晶体。结论：本实验得到了一种快速高效获得对叶百部碱的方法。

英文摘要：

Objective：To obtain a quick and efficient way to get tuberostemonine by optimizing key factors in this process. Methods ： An HPLC - ELSD method was employed to determine the tuberostemonine con- tent in Stemona tuberosa extract. The contents of tuberostemonine were compared under different pH val- ues, extract solvent, extract times and crystalline solvent. Results：The optimal purification conditions were as follows ： the acidified ethanol extract was alkalized with ammonia to pH 8, and partitioned with ethyl ac- etate -petroleum ether（ 1：1 ）for one time to obtain the total alkaloid extract（TAE）. The columnar crystals of tuberostemonine could be directly obtained after dissolve the TAE in methanol with the purity more than 98%. Conclusion ：A rapid and efficient purification method of tuberostemonine was obtained.