中文摘要：

目的：：研究抵抗素对子宫内膜癌细胞增殖的影响及其对子宫内膜癌细胞PI3 K/Akt信号传导通路的作用。方法：CCK-8法检测不同浓度抵抗素（0 ng/ml、10 ng/ml、50ng/ml、100ng/ml、200ng/ml）对Ishikawa 和 KLE 两种子宫内膜癌细胞系增殖的影响。Western blot法检测不同浓度抵抗素作用下PI3 K/Akt信号通路的变化及PI3 K/Akt信号通路抑制剂对抵抗素活化PI3 K/Akt信号通路作用的影响。结果：抵抗素能促进两种子宫内膜癌细胞系Ishikawa和KLE的生长，并呈时间和浓度依赖性。抵抗素可促进两种子宫内膜癌细胞系中PI3 K/Akt信号通路的激活。 PI3 K/Akt信号通路抑制剂LY29004可减弱抵抗素诱导的Akt磷酸化水平。结论：抵抗素能促进子宫内膜癌细胞增殖，这种作用可通过激活PI3 K/Akt信号通路来实现。

英文摘要：

Objective：To determine the effects of resistin on endometrial cancer cell proliferation and its effect on PI3K/Akt signal pathway. Methods：The proliferation impact of different concentrations of resistin on two types of endometrial cancer cells Ishikawa and KLE was confirmed by CCK-8 method. Western blot was used to detect the effect of resistin, LY29004,a PI3K/Akt inhibitor,on the changes of PI3K/Akt signal pathway in the endometrial cancer cells. Results：（1）Resistin significantly enhanced the proliferation of endometrial carci-noma cells in a time and concentration-dependent manner. （2）Resistin can promote the activa-tion of endometrial cancer cellˊs PI3K/Akt signaling pathway. Conclusion：Resistin can promote the proliferation of endometrial carcinoma cells,this effect may be accomplished by the activa-tion of PI3K/Akt signaling pathway.