中文摘要：

目的用实时荧光定量聚合酶链反应（FQ-PCR）方法检测不同亚型干扰素α（IFN-α2b、IFN-α2a、IFN-α1b）对HepG2细胞内信号传导分子STAT1 mRNA表达的影响。方法1000IU／ml IFN-α2b、IFN-α2a、IFN-α1b分别作用于HepG2细胞4、8、16、24h后，用RT-PCR的方法扩增STAT1目的片段，用T-A克隆方法构建定量的标准模板，并用荧光定量PCR方法观察IFN-α2b、IFN-α2a、TFN-α1b作用后HepG2细胞内STAT 1mRNA的表达水平。结果经IFN-α2b、TFN-α2a、IFN-α1b处理后，HepG2细胞内的STAT 1mRNA水平较未用IFN-α的空白对照组明显上调，并于IFN-α处理8h后STAT 1 mRNA水平达高峰。此时，IFN-α2b、IFN-α2a和IFN-α1b的STAT1 mRNA水平（×10^7copies／ml）依次为3．59±0．25、2．73±0．43和4．85±0．55，三者之间存在显著性差异。结论TFNN-α作用于HepG2细胞后，IFN-α1b STAT 1mRNA的表达水平最高，TFN-α 2b次之，IFN-α 2α最弱。间接说明IFN-α1b的抗HBV活性较强，IFN-α2b次之，IFN-α2a较弱。

英文摘要：

Objective To detect the effects of interferon-alpha subtypes（IFN-α 2b, IFN-α 2a and IFN-α 1b）on signaling transduction molecule STAT1mRNA expression in HepG2 cells by realtime fluorescence quatitive PCR （FQ-PCR）. Methods After HepG2 cells were treated with 1 000 IU/ml IFN-α 2b, IFN-α 2a or IFN-α 1 b for 4,8,16,24 h, respectively,we constructed the quantitative standard template with T-A clone methods using the conventional RT-PCR to amplify STAT1 gene from cultured HepG2 cells. STAT1 mRNA expression after pre-treatment with IFN-α 2b, IFN-α 2a or IFN-α 1b was determined with FQ-PCR Results HepG2 cells possessed relatively low basal mRNA levels of STAT1, and their expression was greatly up-regulated by IFN-α. STAT1 rnRNA level reached the maximum after treatment with IFN-α,at which,STAT1 mRNA levels（ ×10^7 copies/ml） were 3.59±0. 25,2. 73±0.43 and 4.85±0. 55 after treated with 1FN-α 2b,IFN-α 2a or IFN-α 1b, respectively. It showed statistical difference among the three groups. Conclusion STATlmRNA expression level was in an order of IFN-α 1b〉IFN-α 2b~IFN-a 2a aftre pre-treatmented with IFN-a, suggesting that the antiviral effect of IFN-α 1b is stronger than that of IFN-a 2b and IFN-α 2a.