中文摘要：

目的： 建立一种同时测定香蜂花药材中迷迭香酸、咖啡酸和咖啡酸乙酯含量的RP-HPLC分析法。方法： Hanbon Megres C18色谱柱 （4.6 mm×250 mm， 5 μm），流动相A为甲酸-乙腈-水（0.5：20：80），B为甲酸-甲醇-乙腈（0.5：40：60），梯度洗脱（0%-25 min，0%-45% B；25%-30 min，45%-100% B；30%-30.1 min，100%-0% B），流速1.0 mL·min-1，柱温35℃，检测波长330 nm。结果： 迷迭香酸、咖啡酸和咖啡酸乙酯分别在6.0-300.0，0.15-7.5 mg·L-1和0.15-7.5 mg·L-1线性关系良好，平均加样回收率（n=6）分别为99.48%，99.56%，101.7%。结论： 该方法准确，简便，适用于香蜂花药材的质量分析检验。

英文摘要：

Objective： The purpose of this study is to simultaneously determine the content of rosmarinic acid, caffeic acid and ethyl caffeate in Melissa officinalis by RP-HPLC. Method： Hanbon Megres C18 column （4.6 mm×250 mm, 5 μm） was adopted. The mobile phase consisted of formic acid-acetonitrile-water （0.5：20：80） and formic acid-methanol-acetonitrile （0.5：40：60） with gradient elution program（0-25 min, 0%-45% B;25-30 min, 45%-100% B;30-30.1 min,100%-0% B）. The flow rate was 1.0 mL·min-1. The column temperature was set at 35℃ and the detection wavelength was 330 nm. Result： The linear ranges of rosmarinic acid, caffeic acid and ethyl caffeate were 6.0-300.0, 0.15-7.5 and 0.15-7.5 mg·L-1 respectively. The average recoveries（n=6） for the sample preparation of the markers were 99.48%,99.56%, and 101.7% respectively. Conclusion： The quantitative method for rosmarinic acid, caffeic acid and ethyl caffeate by HPLC can provide the basis for quality control of M. officinalis.