中文摘要：

本研究利用叠氮化钠（NaN3）对黑麦草品种蓝天堂进行诱变处理。经过M2筛选和M3鉴定,得到农艺性状发生明显变异的突变体60株,并从中选择了2个穗部突变体进行分子鉴定。结果表明,在M2代株系中,共有60株具有稳定的变异性状,其中穗部性状发生变异的植株最多,共21株。在M3代株系中,比较2个穗部突变体与对照的表型发现：对照只有1个顶端穗,但经诱变剂处理后,有2个突变体出现多个顶端穗,其中1个突变体有5个二级分枝。对照和2个突变体在100个引物中有45个引物能扩增出带,其中3个引物能够区分对照与突变体1和2。因此,对照蓝天堂黑麦草和2个M3突变体之间在DNA水平上存在差异。

英文摘要：

The ryegrass（Lolium multiflorum） variety ＂Blue Heaven＂ were treated by sodium azide（NaN3）.After selecting from M2 generation and identification of M3 generation,we obtained 60 mutants with the most significant variation traits.Two panicle mutants were chosen to identify by molecular markers.100 RAPD（randomly amplified polymorphic DNA） random primers were used to analyze check（CK） and 2 panicle mutants.The results showed that 60 plants with stable variation traits were obtained from M2 mutant lines.21 panicle mutants were obtained and it was the most among these mutant types.2 panicle mutants were compared with CK.Only one top spike on CK,2 panicle mutants have more top spikelets.And mutants No.2 and CK have not secondary branch per panicle.Therefore,mutant No.1 has 5 secondary panicle braches.45 RAPD primers can be amplified among the three materials.In addition,the three materials can be differentiated by 3 RAPD primers.So there were difference between CK and the 2 panicle mutants on molecular level.