中文摘要：

应用第3代携带CMV启动子-EGFP报告基因表达元件的重组慢病毒,脂质体法转染293T细胞,调整转染条件进行了平行比较从而获得最优条件组合,得到的包装病毒感染鸡、鸭胚成纤维细胞并计算表达效率。结果表明4种质粒（pLP1、pLP2、p-VSVG、pll3.7）以6.5∶2.5∶3.5∶7.5比例组成混合液转染效率最高,而此条件下提高脂质体-DNA转染复合物浓度达到1∶2时包装病毒滴度达到峰值。用包装病毒感染鸡胚和鸭胚成纤维细胞的效率分别为12%和8%。试验结果证实重组慢病毒可以成功转染家禽细胞,对于进一步探索慢病毒对家禽干细胞的转染和转基因家禽的获得提供了试验依据。

英文摘要：

The third generation lentiviral system constructed with CMV promoter-EGFP reporter gene expression element were packaged in 293 Tcells by various combinations of plasmids and liposome to get the optimum level,and by the final transfection efficiency of the recombinant lentivirus was detected by infection efficiency of chicken and duck embryonic fibroblast cells.Results showed that optimal combination of the four plasmids（pLP1,pLP2,p-VSVG,pll3.7）were at a ratio of 6.5∶2.5∶3.5∶7.5mixture and the liposome-DNA concentrations was 1∶2.Packaging lentivirus can transfect CEF and DEF cells and get 12% and 8% efficiency respectively.The results showed that the recombinant lentiviral vector can transfect poultry cells.It provides the experimental basis for further exploration of poultry stem cells and transgenic poultry.