中文摘要：

减小质量传递效应影响是提高SPR（surface plas-mon resonance）阵列检测一致性的有效途径。该文在理论分析的基础上,提出通过改变流体池结构来改善质量传递效应的影响,并设计4种不同流体池结构,利用Ansys中的FLUENT模块进行有限元仿真。仿真结果表明,与传统的流体池相比,用改变结构的流体池检测得到的动力学常数ka、kd的一致性分别提高了16.9%和23.8%以上。根据仿真结果制作了3种微流体池,利用阵列式SPR生物分析仪对smx分子与其抗体的相互作用进行检测。结果表明：与传统型流体池相比,台阶形和斜坡形流体池动力学常数ka的一致性分别提高了32.3%和42.8%,台阶形和斜坡形流体池动力学常数kd的一致性分别提高了55.8%和35.4%,质量传递效应影响的改善效果明显。这些结果可以用于指导微流体池的设计,为实现高精度的SPR阵列检测创造条件。

英文摘要：

The uniformity of SPR （surface plasmon resonance） array detection can be effectively improved by reducing the mass transport effect. The structures of flow cells were varied based on theoretical analyses to reduce the mass transport effect. Four flow cells with different structures were designed and simulated using the finite element analysis performed by the FLUENT module of Ansys software. Simulation results showed that the uniformity of kinetic constants ka and kd detected with the optimized flow cell are increased by 16.9% and 23.8% compared with the traditional one. Three types of flow cells were fabricated and applied in an SPR array detection instrument to detect the reaction of sulfamethoxazole and its antibody. Experimental results demonstrate that the uniformityof kinetic constants ka detected by the flow cells with triangle and slop structures is increased by 32.3% and 42.8%, with that of kd increased by 55.8% and 35.4%. The mass transport effect is obviously reduced. These results can be applied to the design of flow cells for high precision SPR array detection instruments.