中文摘要：

目的：探讨早孕蜕膜基质细胞培养液（DSCM）对滋养细胞增殖、凋亡以及浸润能力的影响及对浸润功能的作用机制。方法：原代分离、培养并鉴定人早孕蜕膜基质细胞,收集并制备成不同浓度的DSCM,采用噻唑兰（MTT）、Annexin V-FITC、Transwell方法检测不同浓度的DSCM对滋养细胞增殖、凋亡、浸润能力的影响,采用RT-PCR及明胶酶谱法检测滋养细胞基质金属蛋白酶（MMPs）的改变。结果：得到纯度大于90%的蜕膜基质细胞。随着DSCM的浓度增高（5%DSCM,20%DSCM,50%DSCM,100%DSCM）细胞的吸光度值增高、凋亡率减少、浸润细胞数增多（P〈0.05）。不同浓度的DSCM组MMP-2及MMP-9 mRNA、蛋白的变化与对照组相比差异均有统计学意义（P〈0.05）。结论：早孕DSCM可以促进滋养细胞增殖,抑制凋亡,并通过上调滋养细胞对MMP-2与MMP-9的表达来促进其浸润功能。

英文摘要：

Objective：To investigate the impact of conditioned medium of decidual stromal cell culture（DSCM） on proliferation, apoptosis and invasion of trophoblast cells. Methods.lsolution, culturing and indentifying the purity of decidual stromal cells from healthy women of early pregnancy to prepare different concentrations of DSCM （5% ,20% ,50% and 100% ） ;Proliferation was evaluated by MTT assay; Cell apoptosis was examined by flow cy- tometry using annexin V-FITC/PI staining; Cell invasion was assayed using a Transwell chamber; RT-PCR and gelatin zymography were used to explore the mechanism of cell invasion. Results： Using this method we obtained a purify over 90% of decidual stromal cells. With the concentration of DSCM increased（5% ,20% ,50%, 100% ）, the OD value of the MTT assay has elevated and the rate of apoptosis declined,the infiltrating cell increased as well （ P 〈 0.05 ） ;Compared with control group, DSCM treatment led to a significant increase in the mRNA and pro- tein expression of MMP-2/9（ P 〈0.05）. Conclusions：Decidual microenviroment may promote trophoblast prolif- eration and inhibite its apoptosis besides could enhance its ability of invasion by altering the expression of MMP-2 and MMP-9.