中文摘要：

本试验旨在以“无菌鸡”为模型，研究添加鸡源性乳酸菌和盲肠内容物后，对仔鸡肠道发育、黏膜形态及免疫器官发育的影响。试验选用无特定病原体（ SPF）种蛋80枚，经无菌孵化后，选择健康仔鸡60羽，分3组（每组20羽），转入3台无菌隔离器内饲养，并分别饲喂：无菌饲粮（ CT组）、无菌饲粮＋乳酸菌液（ LB组）、无菌饲粮＋鸡盲肠内容物（ CC组）；饲养21 d后试验结束，于试验7、14、21日齡从各组取6只进行屠宰，测定鸡肠道各段长度和免疫器官重量，并对肠道黏膜形态显微观察。结果显示：饲喂乳酸菌或盲肠内容物的仔鸡各阶段中的空肠长度均高于CT组，盲肠长度和体积均低于CT组的无菌鸡；仔鸡的脾脏指数高于CT组（ P＝0．010）；饲喂乳酸菌或盲肠内容物后仔鸡的十二指肠、空肠和回肠隐窝深度降低、绒毛高度／隐窝深度增大。本试验说明，饲喂肠道菌群能促进仔鸡肠道发育，盲肠体积变小；同时促进脾脏的发育，提高机体免疫力。

英文摘要：

The gnotobiotic chicken was selected as a model in this study to investigate the effects of coloniza-tion with chicken-derived Lactobacillus and chicken cecal microbial on intestinal development, mucosal mor-phology and immune organs development of chicks. After 21 days aseptic hatching for 80 specific pathogen free （ SPF） eggs, a total of 60 healthy chicks were transferred to 3 gnotobiotic isolators （ 3 groups and 20 chicks for per group） , and were fed a basal sterile diet （ CT group） , the basal sterile diet＋Lactobacillus culture （ LB group） , the basal sterile diet＋chicken cecal contents （ CC group） . Six chicks were slaughtered from 3 groups at 7, 14 and 21 days of age, the intestinal long mucosal morphology and immune organs weight were meas-ured. The results showed as follows：compared with CT group, the jejunum length of chicks added with Lacto-bacillus or cecal contents was higher, cecal length and volume were lower. The spleen index was significantly higher than that of the CT group （P=0.010）. Compared with gnotobiotic chicks, the crypt depth of duode-num, jejunum and ileum was decreased;the villi height/crypt depth （ V/C） of duodenum, jejunum and ileum was increased for the chicks added with Lactobacillus or cecal contents. It is concluded that intestinal flora can promote intestinal development, narrow the cecum volume, while promote the development of spleen, enhance immunity.