不变、解决时间的荧光光谱学被用来学习 Chl 的激动的州的性质一在里面 light-harvestingcomplex II (LHC II ) 从的不同聚集形式一内部潮汐的绿水藻, Bryopsis corticulans,即 LHC II 单体,更整齐并且寡聚物。什么时候任何一个 Chl or Chl b 有选择地激动,在在寡聚物的荧光被证明主要被熄灭 amongChl 的快荧光引起的 Chl 的观察减少一分子,而非由在 Chl b-to-Chl 的减少汗衫刺激转移效率。LHC II 的三种形式在所有解决时间的荧光动力学识别了的微微秒分析二个指数的腐烂部件:从 Chl 的荧光排放发源的一个长生活的部件(4.1 -4.7 ns ) 一,并且突然生活的(135 - 540 ps ) 从在 Chl 之中的汗衫刺激的快速的使平衡一分子。刺激使平衡的时间常数是在寡聚物的 135 ps, 520 ps 在更整齐并且在单体的 540 ps。Theseresults 暗示在寡聚物形式的 LHC II 内在地能熄灭 Chl 刺激,可能与经由在 Bryopsis corticulans 改变 LHC IIaggregation 的度的 PS II 的相片保护有关的机制。
Steady-state and time-resolved fluorescence spectroscopies have been used to study the excited state properties of Chl a in different aggregation forms of light-harvesting complex Ⅱ (LHC Ⅱ) from an intertidal green alga, Bryopsis corticulans, i.e. LHC Ⅱ monomer, trimer and oligomer. When either Chl a or Chl b was selectively excited, the observed decrease in Chl a fluorescence in the oligomer is proved to be caused mainly by the fast fluorescence quenching among Chl a molecules, rather than by the decrease in Chl b-to-Chl a singlet excitation transfer efficiency. Analyses of the picosecond time-resolved fluorescence kinetics identified two exponential decay components in all of the three forms of LHC Ⅱ: a longer-lived component (4.1 -4.7 ns) originating from fluorescence emission of Chl a, and a shorter-lived one (135-540 ps) from the rapid equilibration of singlet excitation among Chl a molecules. The time constant of excitation equilibration is 135 ps in oligomer, 520 ps in trimer and 540 ps in monomer. These results imply that LHC Ⅱ in oligomer form is inherently able to quench Chl a excitation, a mechanism which may be related to the photoprotection of PS Ⅱ via changing the degree of LHC Ⅱ aggregation in Bryopsis corticulans.