中文摘要：

Jingzhaotoxin --(JZTX-I ) 我从蜘蛛 Chilobrachysjingzhao 的毒液净化了新奇神经毒素优先地由绑定在激活正在禁止心脏的钠隧道到受体地点 3。在水的答案的这毒素的结构是调查 using2-D ~ 1H-NMR 技术。在 JZTX-I 的~ 1H-NMRspectra 的质子回声的完全的顺序特定的任务被分析一系列 2-D 系列获得，包括DQF舒适， TOCSY andNOESY 系列，在 H_2O 和 D_2O.All 脊梁质子除了 Gln4 并且非常，95%theside链质子被d_(高山汉)识别，d_(高山汉)，d_(打赌一)并且在 NOESY 光谱的d_( NN )连接。这些研究为 JZTX-I.Furthermore 的答案符合构造的进一步的决心提供一个基础， JZTX-I 的二级结构与 Trp7-Cys9 从主要一张短搁浅三元组的反平行的贝它表的 NMR data.Itconsists 被识别， JZTX-I 的二级结构的 Phe20-Lys23 andLeu28-Trp31.The 特征类似于那些 ofhuwentoxin --我( HWTX-I )并且 hainantoxin-IV ( HNTX-IV )，其在答案的结构以前被报导了。

英文摘要：

Jingzhaotoxin-I （JZTX-I） purified from the venom of the spider Chilobrachys jingzhao is a novel neurotoxin preferentially inhibiting cardiac sodium channel inactivation by binding to receptor site 3.The structure of this toxin in aqueous solution was investigated using 2-D ^1H-NMR techniques. The complete sequence-specific assignments of proton resonance in the ^1H-NMR spectra of JZTX-I were obtained by analyzing a series of 2-D spectra, including DQF-COSY, TOCSY and NOESY spectra, in n20 and D20. All the backbone protons except for Gin4 and more than 95% of the side-chain protons were identified by dαN,dαδ, dβN and dNN connectivities in the NOESY spectrum. These studies provide a basis for the furtherdeter mination of the solution conformation of JZTX-I. Furthermore, the secondary structure of JZTX-I was identified from NMR data. It consists mainly of a short triple-stranded antiparallel β-sheet with Trp7-Cys9, Phe20-Lys23 and Leu28-Trp31. The characteristics of the secondary structure of JZTX-I are similar to those of huwentoxin-I （HWTX-I） and hainantoxin-IV （HNTX-IV）, whose structures in solution havepre viously been reported.