中文摘要：

[目的]利用小动物成像技术,探讨华蟾素对裸小鼠Bx PC3-luc2胰腺癌的抗肿瘤作用。[方法]Bx PC3-luc2胰腺癌细胞接种于24只雄性裸小鼠皮下7d后,分成3组：即空白组、华蟾素组[6g·kg-1华蟾素（生药）]、顺铂组（2mg·kg-1DDP）,每组8只,连续腹腔注射给药14d;用游标卡尺和活体生物发光成像仪分别在第7d和第14d检测各组裸小鼠胰腺癌肿瘤生长情况,并在第14d处死小鼠,取实体瘤称重。[结果]与空白组比较,华蟾素组体重无明显变化,肿瘤体积和重量明显减小,肿瘤光子量明显降低,在给药第7d和14d时,肿瘤光子量抑制率分别为30.11%和63.81%,但6g·kg-1华蟾素作用弱于2mg·kg-1顺铂,而华蟾素对小鼠体重无明显变化,具有一定的优势。[结论]华蟾素具有较好的抑制裸小鼠Bx PC3-luc2胰腺癌的作用。

英文摘要：

[Objective] To investigate the effects of inhibiting pancreatic cancer of Cinobufotalin by bioluminescent imaging（BLI） in the Bx PC3-luc2 cell xenograft nude mice.[Method] Twenty-four nude mice were subcutaneously injected Bx PC3-luc2 pancreatic cancer cells. After seven days, tumor-bearing mice were divided into three groups： blank group, Cinobufotalin group（6g·kg-1, Cinobufotalin） and positive drug group（2mg·kg-1DDP）, eight mice for each group and continuous intraperitoneal injection of 14 d. The tumor growth of pancreatic cancer in nude mice was detected by using the card scale and living creature luminescence imaging instrument at 7th and 14 th days, and the animals were sacrificed at 14 th day, and the solid tumor was weighed. [Result]Tumor bearing nude mice with injection of Cinobufotalin had no significant changes in body weight compared with the control group, while tumor weight and volume were significantly reduced; Tumor photon was also significantly reduced in nude mice treated with Cinobufotalin at after administration of 7th and 14 th days, the inhibition rates of tumor photon were 30.11% and 63.81% respectively. But 6g·kg-1Cinobufotalin was weaker than 2mg·kg-1DDP. The effect of Cinobufotalin did not change significantly for body weight in mice, had certain advantages. [Conclusion] Cinobufotalin has a good inhibition of Bx PC3 pancreatic cancer in nude mice.