中文摘要：

目的：观察奥沙利铂诱发外周神经病变时大鼠的疼痛行为学改变以及降钙素基因相关肽（calcitonin gene‐re‐lated peptide ，CGRP）的表达情况。方法20只Wistar大鼠随机分成对照组和实验组，实验组予以腹腔注射奥沙利铂（20 mg／kg）造模。分别在造模前、造模后6、24、72 h和7 d ，检测大鼠机械性痛阈和冷痛阈，应用Western blot及免疫组化检测脊髓背根神经节中CGRP蛋白的表达。结果造模后6 h大鼠机械性痛阈较正常对照组降低[（11.6±2.2）g vs.（13.9±3.7）g]，并随着观察时间的延长而下降，在72 h降至低点（3.9±1.4）g ，一直持续到7 d ，冷刺激缩足潜伏期也呈现同样趋势的变化。奥沙利铂化疗后，大鼠脊髓背根神经节中CGRP表达明显升高，蛋白表达上调，并且与机械痛阈呈正相关。结论 CGRP在奥沙利铂诱导的外周神经病变大鼠中枢敏化中发挥重要促进作用。

英文摘要：

Objective To observe the pain behavior of rats with Oxaliplatin‐induced peripheral neuropathy（OXIPN）and cal‐citonin gene related peptide（CGRP）expression.Methods Twenty Wistar rats were randomly divided into 2 groups ：normal con‐trol group and model group.Oxaliplatin（20 mg/kg）was injected intraperitoneally to the rats in the model group.Mechanical pain threshold and cold allodynia were detected before and 6 ,24 ,72 h and 7 days after modeling ,respectively.Western blot and im‐munohistochemical method were employed to measure the expression of CGRP in dorsal spinal root ganglion of control group and model group.Results The mechanical pain threshold of modal group rats decreased 6 h after injection of Oxaliplatin as compared with the normal group[（11.6 ± 2.2）g vs. （13.9 ± 3.7）g] ,and decreased with prolongation of time ,reduced to the lowest at 72 h[（3.9 ± 1.4）g]and maintained to day 7 ,and the same trend was found in cold chemical allodynia test.CGRP ex‐pression of dorsal root ganglion increased significantly in the model group rats ,and there was a significant positive correlation between the expression of CGRP and the degree of mechanical pain threshold.Conclusion CGRP plays an important role in Ox‐aliplatin‐induced central sensitization of rats with peripheral neuropathy.