中文摘要：

alpha-N-acetylgalactosaminidase (高山哈 NAGA ) 罐头 convert 组 A 人红血房间(RBC ) 到组 O。一座新奇高山哈 NAGA 基因被 PCR 从从一件国内临床的样品孤立的 Elizabethkingia meningosepticum 克隆。纯 recombinant 高山哈 NAGA 被遗传工程和蛋白质纯化与 49.6 kD 的一个计算分子获得。高山哈 NAGA 为有一项高特定的活动的终端 alpha-N-acetylgalactosamine 残余是选择的。高山哈 NAGA 能完全移开 1 U (大约 100 mL ) 的 A 抗原组 A (1 ) 或 A (2 ) 在在酸碱值的 1 h 的 RBC 有 1.5 或 0.4 mg recombinant 酶的消费的 6.8 和 25 度 C。变换酶的组 A RBC 没在与单音的同种细胞的反 A 或重量的单位被混合以后粘合 of 组 A， B， AB 和 O。除了土佬的另外的血型抗原没有零钱。FCM 分析显示出那 A 抗原和 A (1 ) 当 H 抗原增加了时，抗原消失了。它显示了那座高山哈 NAGA 成功地把人的血型 A RBC 变换成普遍没有土佬不兼容的输送反应的风险的 transfusable 组 O RBC。这座高山哈 NAGA 对生产通用 RBC 增加临床的输送安全，改进 RBC 供应，并且到减少，输送花费并且支持在紧急情况的情况下的输送服务合适。

英文摘要：

α-N-acetylgalactosaminidase （αNAGA） can convert group A human red blood cells （RBCs） to group O. One novel αNAGA gene was cloned by PCR from Elizabethkingia meningosepticum Isolated from a domestic clinical sample. Pure recombinant αNAGA was obtained by genetic engineering and protein purification with a calculated molecule of 49.6 kD. αNAGA was selective for terminal α-N-acetylgalacto- samine residue with a high specific activity, αNAGA could completely remove A antigens of 1 U （about 100 mL） group A1 or A2 RBCs in 1 h at pH 6.8 and 25℃ with s consumption of 1.5 or 0.4 mg recombinant enzyme. Enzyme-converted group A RBCs did not agglutinate after being mixed with monoclonal snti-A or sere of groups A, B, AB and O. Other blood group antigens except ABO had no change. FCM analysis showed that A antigens and A1 antigens disappeared while H antigens increased. It indicated that αNAGA successfully converted human blood group A RBCs to universally transfusable group O RBCs without the risk of ABO-incompaUble transfusion reactions. This αNAGA was suitable for producing universal RBCs to increase clinical transfusion safety, improve the RBCs supply, and to decrease transfusion cost and support transfusion service in case of emergency.