中文摘要：

目的：研究乙肝病毒X蛋白结合蛋白（HBXIP）在人肝癌细胞中的表达和定位。方法：蛋白印迹法检测HBXIP在人肝癌细胞系中蛋白和基因的表达,激光共聚焦扫描显微镜检测内源HBXIP在BEL7402、HepG2肝癌细胞中的定位。结果：HBXIP在人肝肿瘤细胞系中蛋白和基因表达水平增高,激光共聚焦扫描显微镜观察发现HBXIP主要定位于BEL7402、HepG2细胞核中。结论：HBXIP在人肝癌细胞系中高表达,主要定位于肝癌细胞核内,可能是人肝癌重要的调节因子。

英文摘要：

Objective To investigate the expression and localization of hepatitis B vims X protein binding protein （HBXIP） protein in human liver cancer. Methods ： Immunoblot analysis for exogenous HBXIP expression from human hepatocellular carcinoma cell lines. The endogenous localization of HBXIP in BEL7402 and HepG2 cells was observed by using laser scanning confocal microscopy. Results ： HBXIP protein expression was high in human liver cancer cells.The human HBXIP protein was mainly localized in the nucleus by laser scanning confocal microscopy. Conclusion：HBXIP was highly expressed in human liver cancer cell lines and localized mainly in the nucleus,which may be an important regulator in human liver cancer.