中文摘要：

以海南坡鹿外周血白细胞总RNA为模板,利用RT-PCR技术,获得海南坡鹿MD2全长cDNA,并对其进行较系统的生物信息学分析,内容包括序列特征分析、同源性分析、编码蛋白质理化性质及结构预测。结果表明：海南坡鹿MD2的CDS序列长度为486 bp,编码160个氨基酸,与黄牛、人、欧洲兔、褐家鼠的核苷酸序列同源性分别为98.55%,77.43%,78.47%和74.74%。该基因编码的蛋白质相对分子质量约为19×10^3,理论等电点为8.18。对编码蛋白的分子结构预测发现,该蛋白为非跨膜蛋白,其二级结构中,α-螺旋占16.25%,β-折叠片占38.13%,无规则卷曲占45.63%。

英文摘要：

Myeloid differential protein-2（MD2） cDNA of Hainan Eld＇s deer（Cervus eldi hainanus） was amplified by RT-PCR and the sequences were analyzed.The bioinformatics analysis results indicated that MD2 cDNA ORF contained a 486 bp encoding 161 amino acids;compared to yellow cattle,human,rabbit and mouse,the nucleotide sequence homology of MD2 were 98.55%,77.43%,78.47% and 74.74%,respectively;relative molecular weight was 19 000,pI was 8.18.The molecular structure prediction results suggested that the protein was non-transmembrane protein,which contained 16.25% α-helix,38.13% β-meander,45.63 % random coil.