中文摘要：

人C组5型腺病毒（Ad5）载体能够有效感染上皮来源的细胞，但对造血细胞的感染效率很低，限制了其在造血调控基础研究以及血液病基因治疗中的应用。为了建立高效感染血液细胞的新型靶向性腺病毒载体系统，对5型腺病毒载体的纤维顶球进行了改造，以AdEasy系统为基础，应用递归PCR的方法人工合成人B组11p型腺病毒的部分纤维（fiber）基因，采用一系列分子生物学方法将其替换AdEasy骨架质粒中的人5型腺病毒的fiber基因，得到新的腺病毒骨架质粒命名为pAdEasy-1／F11p，应用带有GFP报告基因的穿梭质粒pShuttle-GFP与AdEasy-1／F11p腺病毒DNA在BJ5183细菌内重组得到重组腺病毒质粒，将其转染293细胞获得重组腺病毒，命名为Ad5F11p-GFP。以Ad5-GFP作对照，同时感染K562、U937等白血病细胞系，流式细胞仪检测GFP的表达。初步检测结果显示：在10MOI时，Ad5F11p-GFP能够有效感染K562、U937等白血病细胞系，感染细胞效率〉90％，对照Ad5-GFP感染细胞效率〈30％，这表明改建后的腺病毒AdEasy-1／F11p可以高效介导基因转移到血液细胞，是一种很好的血液细胞靶向性腺病毒载体。

英文摘要：

The adenovirus subgenus C serotypes 5 （Ad5） vectors which have been used to transduce epithelial cells have very limited ability to infect hematopoietic cells. For feasible generation of fiber-retargeted adenoviral vectors, we have modified the versatile AdEasy system with a chimeric fiber gene encoding the Ad5 fiber tail domain and Ad11p fiber shaft and knob domains. An Ad5-based vector encoding the green fluorescent protein （GFP） gene under the control of the CMV promoter with Ad11p fiber receptor specificity was generated （Ad5F11p-GFP）. The Ad5F11p-GFP vector-mediated gene transfer efficiency for some committed hematopoietic cell lines such as myeloblasts （K562） and monocytes （U937） was evaluated using flow cytometry and compared to that of Ad5-GFP, which also encodes the GFP gene under the control of the CMV promoter. The results showed that these cell lines were superiorly transduced by the Ad5F11p-GFP vector at the same MOI （multiplicity of infection, MOI） compared with the Ad5-GFP vector, more than 90% tested cells were infected by Ad5F11p-GFP adenov, irus while less than 30% cells were infected by Ad5-GFP at 10 MOI.