中文摘要：

为明确枯草芽孢杆菌（Bacillus subtilis）G87抗菌蛋白的生物活性,采用菌丝生长、分生孢子萌发和芽管伸长抑制的方法,研究其对稻瘟病菌（Magnaporthe grisea）和稻恶苗病菌（Gibberella fujikuroi）的抑制作用。结果表明：抗菌蛋白能明显抑制2种病菌菌丝生长、分生孢子萌发和芽管伸长。1.20mg·mL-1抗菌蛋白对稻瘟病菌菌落直径和菌丝干重的抑制率分别达60%和80%以上;2.40mg·mL-1抗菌蛋白对稻恶苗病菌分生孢子萌发和芽管伸长的抑制率均达60%以上。抗菌蛋白还能显著破坏2种病菌菌丝形态,使菌丝细胞畸形膨大、原生质浓缩和外渗、细胞壁破损以及菌体崩溃等。因此,枯草芽孢杆菌G87抗菌蛋白具有重要的抗菌生物活性。

英文摘要：

In order to clarify the biological activity of antifungal protein from Bacillus subtilis G87,its inhibition to Magnaporthe grisea and Gibberella fujikuroi was studied.The results showed that the antifungal protein exhibited inhibitory activity on mycelial growth,conidial germination and germ-tube elongation of rice pathogenic fungi mentioned above.At the concentration of 1.20 mg·mL-1 for the antifungal protein,the inhibiting rates were above 60% and 80% against the colony growth and the dry mycelial weight of M.grisea,respectively.In the presence of 2.40 mg·mL-1 antifungal protein,the inhibiting rates toward the conidial germination and germ-tube elongation of G.fujikuroi were both over 60%.Moreover,the antifungal protein was demonstrated to have a destructive effect on hyphal shape of the two pathogenic fungi,with hyphal cell distortion,protoplasm concentration and leakage,tumescence,cell wall rupture,and collapse.Therefore,B.subtilis G87 antifungal protein has an important antifungal biological activity.