中文摘要：

我们从 Vibrio anguillarum M3 紧张克隆并且定序象 prtV 一样基因。这 prtV 基因编码 918 氨基酸的通常认为的蛋白质，并且对 V 高度相应。cholerae prtV 基因。我们发现一个 prtV 插入异种种类为四 glycosidases 在明胶琼脂，对 azocasein 的更低的朊酶活动，和更低的活动上显示了更低的 gelatinase 活动。这个 prtV 异种种类也在它的细胞外的产品为二 esterases 增加了活动，由 API ZYM 系统分析了。另外， prtV 异种种类在比目鱼之类展出了减少的生长比目鱼之类红血球上的肠的粘液和减少的 hemolytic 活动。感染实验证明 prtV 异种种类的 LD50 由至少 1 根木头增加了与相比在比目鱼之类鱼野类型。我们建议 prtV 在 V 的致病起一个重要作用。anguillarum。关键词 Vibrio anguillarum - prtV -解朊作用-溶血-粘液- LD50 由中国( 973 程序)(号码 2006CB101803 )的国家基本研究程序支持了，中国(号码 30871935 )的国家自然科学基础，并且国家高技术研究和开发编程序( 863 程序)(号码 2003AA622070 )

英文摘要：

We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids, and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the API ZYM system. In addition, the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD50 of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V, anguillarum.