克隆和分析了2个小菜蛾的储存蛋白基因——PxAJSP-1和PxBJHSP-2.其中,PxAJSP-1属于芳基贮存蛋白,而PxBJHSP-2属于富甲硫氨酸储存蛋白.进化树分析表明,它们在同一目昆虫中相对保守.利用实时荧光定量PCR分析其表达模式,发现PxAJSP-1和PxBJHSP-2在4龄幼虫和蛹期高表达,且在脂肪体中的表达量远高于其他组织.
Two storage protein genes in Plutella xylostella, PxAJSP-1 and PxBJHSP-2, were cloned and characterized. The results showed that PxAJSP-1 belongs to the arylphorin-like protein and PxBJHSP-2 belongs to the methionine-rich protein. Phylogenetic tree analyses revealed that both genes were relatively conservative among the insects of the same order. The expression pattern analyses by quantitative real-time PCR(qPCR)showed that PxAJSP-1 and PxBJHSP-2 were highly expressed in the fourth-instar larvae and pupae, respectively. And their expressions predominated in fat body than other tissues.