中文摘要：

为了建立测定术苦芩颗粒中黄芩苷、苦参碱含量的反相高效液相色谱法,采用Wonda Sil（R） C18柱（4.6 mm×250 mm,5μm）色谱柱,黄芩苷以甲醇-0.4％磷酸溶液（用三乙胺调节Ph值至3.0）（52∶48,V/V）为流动相,检测波长为274 nm.苦参碱以乙腈∶甲醇∶0.2％磷酸溶液（用三乙胺调节Ph值至7.0）（22∶13∶65,V/V）为流动相,流速为1.0 Ml/min,检测波长220 nm;柱温30℃.术苦芩颗粒中黄芩苷和苦参碱分别在浓度为10 μg/Ml～150 μg/Ml和10 μg/Ml～400 μg/Ml范围内与峰面积均呈良好线性关系,r=0.99991和r=-0.99994,平均加样回收率分别为99.32％和98.37％,其相对标准偏差（RSD）分别为0.625％和1.206％.用上述方法分别测定黄芩苷和苦参碱含量条件简单、稳定、快速,可用于检测术苦芩颗粒的质量控制标准.

英文摘要：

In order to establish a method for the determination of baicalin and matrine content in Zhukuqin granules. Chromatography was performed on a Wonda Sil C 18 column（ 4.6 mm×250 mm05 μm）. and acetonitrile-methanol-0.2% phosphoric acid solution （V： V = 52：48） as the mobile phase of baicalin was used at the wavelength of 274 nm and acetonitrile-methanol-0.2% phosphoric acid solution （V：V：V=22：13 ： 65） as the mobile phase of matrine was used at the wavelength of 220 nm with 10 IzL of the injection volume, at the flow rate of 1.0 mL/mL and column temperature of 30 ~C.The results showed that the calibration curve of baicalin was linear i.n the rang of 10 trg/mL ～ 150 μg/mL（r=0.99991 ）. and the average recovery rate was 99.32%（RSD=0.625%）. And the calibration curve of matrine was linear in the rang of 10 μg/mL ～400 μg/mL（r=0.99994）. and the average recovery rate was 98.37%（RSD=1.206%）. The method was feasible and stable for the determination of baicalin and matrine content in ZhuKuqin granules as the quality control standard.