以6头装有永久瘤胃瘘管的小尾寒羊为试验动物,用尼龙袋法测定了2种不同生育期豆科牧草苜蓿和白三叶在绵羊瘤胃降解前后氨基酸的变化差异.结果表明,1)2种豆科牧草总氨基酸含量总体上随生育期呈下降趋势,即在分蘖期最高,成熟期最低.总氨基酸/粗蛋白的高峰值都出现在开花期;2)2种豆科牧草的天冬氨酸.谷氨酸.甘氨酸.脯氨酸.酪氨酸.丝氨酸.精氨酸.苏氨酸.赖氨酸和苯丙氨酸在瘤胃中较易降解(犘〈0.05或犘〈0.01);丙氨酸.缬氨酸.蛋氨酸.胱氨酸.异亮氨酸.亮氨酸在瘤胃中有比较好的抗降解作用(犘〉0.05);3)随着生育期的推进,成熟牧草中的各种氨基酸在瘤胃中越不易降解.
Six sheep fitted with permanent ruminal cannulas were used to determine the degradation profiles of amino acids (AA) from two legumes at different maturities after 6 or 16 h ruminal incubation using a nylon - bag technique. 1)With increased maturity of the legumes, the TAA content decreased: The highest values were in the tillering period and the lowest at maturity. 2)After ruminal incubation, Asp, Glu, Gly, Pro, Tyr, Ser, Arg, Thr, Lys and Phe were degraded in the rumen to a greater extent, than Ala, Val, Met, Cys, Ile, and Leu and the highest concentration was after 16 h ruminal incubation. 3)With increased maturity of legumes, all legume AA became less degradable in the rumen.