瞄准:为了比较地学习修改 gelatinizedly 的壳聚糖的预防效果,腹膜粘连上的电影在老鼠由四个不同因素导致了。方法:壳聚糖被胶凝化学上修改,并且在厚度转变为 60 妈妈的电影,并且消毒。200 只 Sprague-Dawley 老鼠随机被划分成五个组,假冒操作的组(组 A ) ,导致创伤的粘附组(组 B ) ,净化的导致滑石的粘附组(组 C ) ,脉管的导致结扎的粘附组(组 D ) ,和导致感染的粘附组织(组 E ) 分别地。在每个组,老鼠在阑尾的盲肠与不同导致粘附的方法被对待然后被划分成控制和试验性的亚群。阑尾的浆液的膜表面在试验性的亚群被这些电影盖住,并且没有电影在控制亚群被使用。在处理的 2 和 4 wk 以后,腹的洞被再开,粘合剂严厉根据 Bhatia 的方法盲目地被分级。阑尾的盲肠是为羟脯氨酸(OHP ) 的 resected 测量和病理学的检查。结果:粘附严厉和 OHP 水平:在处理的 2 和 4 wk 以后,在试验性的亚群,粘附显著地更轻, OHP 层次是比在组 B 的控制亚群的那些显著地低的(2 wk:0.199 +/- 0.026 对 0.285 +/- 0.041 mug/mg pr, P 【 0.001;4 wk:0.183 +/- 0.034 对 0.276 +/- 0.03 mug/mg pr, P 【 0.001 ) , D (2 wk:0.216 +/- 0.036 对 0.274 +/- 0.040 mug/mg pr, P = 0.004;4 wk:0.211 +/- 0.044 对 0.281 +/- 0.047 mug/mg pr, P = 0.003 ) 并且 E (2 wk:0.259 +/- 0.039 对 0.371 +/- 0.040 mug/mg pr, P 【 0.001;4 wk:0.242 +/- 0.045 对 0.355 +/- 0.029 mug/mg pr, P 【 0.001 ) ,但是在组 A 没有有效差量(2 wk:0.141 +/- 0.028 对 0.137 +/- 0.026 mug/mg pr, P = 0.737;4 wk:0.132 +/- 0.031 对 0.150 +/- 0.035 mug/mg pr, P = 0.225 ) 并且 C (2 wk:0.395 +/- 0.044 对 0.378 +/- 0.043 mug/mg pr, P = 0.387;4 wk:0.370 +/- 0.032 对 0.367 +/- 0.041 mug/mg pr, P = 0.853 ) ;病理学的变化:在组 B,主要病理学的变化是在对待的浆液的膜表面并且在组 D 的 fibroplasias,纤维组织形成?
AIM: To comparatively study the preventive effect of gelatinizedly-modified chitosan film on peritoneal adhesions induced by four different factors in rats. METHODS: Chitosan was chemically modified by gelatinization, and made into films of 60 μm in thickness, and sterilized. Two hundred Sprague-Dawley rats were randomly divided into five groups, Sham-operation group (group A), wound-induced adhesion group (group B), purified talc-induced adhesion group (group C), vascular ligation-induced adhesion group (group D), and infection-induced adhesion group (group E), respectively. In each group, the rats were treated with different adhesion-inducing methods at the cecum of vermiform processes and then were divided into control and experimental subgroups. Serous membrane surface of vermiform processes were covered with the films in the experimental subgroups, and no films were used in the control subgroups. After 2 and 4 wk of treatments, the abdominal cavities were reopened and the adhesive severity was graded blindly according to Bhatia's method. The cecum of vermiform processes were resected for hydroxyproline (OHP) measurement and pathological examination. RESULTS: Adhesion severity and OHP level: After 2 and 4 wk of the treatments, in the experimental subgroups, the adhesions were significantly lighter and the OHP levels were significantly lower than those of the control subgroups in group B (2 wk: 0.199 ± 0.026 vs 0.285 ± 0.041 μg/mg pr, P 〈 0.001; 4 wk: 0.183 ± 0.034 vs 0.276 ± 0.03 μg/mg pr, P 〈 0.001), D (2 wk: 0.216 ± 0.036 vs 0.274 ± 0.040 μg/mg pr, P = 0.004; 4 wk: 0.211 ± 0.044 vs 0.281 ± 0.047 μg/mg pr, P = 0.003) and E (2 wk: 0.259 ± 0.039 vs 0.371 ± 0.040 μg/mg pr, P 〈 0.001; 4 wk: 0.242 ± 0.045 vs 0.355 ± 0.029 μg/mg pr, P 〈 0.001), but there were no significant differences in groups A (2wk: 0.141 ± 0.028 vs 0.137 =k 0.026 μg/mg pr, P = 0.737; 4 wk: 0.132 ± 0.031 vs 0.150 ± 0.035 μg/mg pr, P = 0.225) a