中文摘要：

目的：考察人参皂苷Rg。配伍乌头碱对体外培养心衰模型心肌细胞的保护作用。方法：将新生大鼠心肌细胞分为正常对照组、模型组、阳性对照组（去乙酰毛花苷注射液，1×10^-7mol／L）、人参皂苷l沁，组（1×10^-8mol／L）、乌头碱组（1×10^-9mol／L）以及人参皂苷Rg1与乌头碱不同配比的配伍组（1：1、2：1、1：2，V／V）。除正常对照组外，其余各组细胞均以O．8％戊巴比妥钠诱导心肌细胞心衰模型。成模后，各组细胞给予相应药物培养1h，然后检测细胞总三磷酸腺苷（ATP）酶、Ca^2＋-Mg^2＋．ATP酶和Na^＋ -K^＋ -ATP酶活力；检测细胞培养液中酸性磷酸酶（ACP）、乳酸脱氢酶（LDH）活力和脑钠肽（BNP）、肿瘤坏死因子α（TNF-α）含量以及细胞中总糖原含量。结果：与正常对照组比较，模型组细胞总ATP酶和Ca^2＋ -Mg^2＋ -ATP酶活力明显降低，Na^＋ -K^＋ -ATP酶活力明显升高，培养液中ACP、LDH活力及BNP含量均明显升高（P〈O．05）。与模型组比较，各给药组细胞总ATP酶活力显著升高，细胞培养液中LDH活力显著降低，且以人参皂苷Rg。与乌头碱2：1配伍时作用最强；乌头碱组和各配伍组细胞Na^＋ -K^＋-ATP酶活力均显著降低，差异均有统计学意义（P〈0．05）；各给药组细胞培养液中ACP、BNP活力均有降低的趋势，细胞内总糖原含量和培养液中TNF-α含量无显著变化（P〉O．05）。结论：人参皂苷Rg1配伍乌头碱可改善大鼠心衰模型心肌细胞ATP酶活力和膜通透性，调节细胞分泌BNP，对心衰模型心肌细胞有一定保护作用，且以人参皂苷Rg1与乌头碱2：1配伍时作用最强。

英文摘要：

OBJECTIVE： To investigate the protective effect of the compatibilities of ginsenosides Rg1 and aconitine on myocar- dial cell of in vitro cultured heart failure model. METHODS： The myocardial cells of neonate rat were grouped into normal control group, model group, positive control group （Deslanoside injection, 1×10-7 mol/L）, ginsenosides Rg1 group （1×10-8 mol/L）, acon- itine group （1 × 10-9 mol/L） or their compatibilities groups （1 ： 1,2 ： 1,1 ： 2, V/V）. Except for normal control group, other groups were given 0.8% pentobarbital sodium to induce heart failure model of myocardial cells. After modeling, each group was given rele- vant medicine for 1 h, and then the activities of T-ATPase, Ca2＋-Mg2＋-ATPase, Na＋-K＋-ATPase in cells were all detected. The activi- ties of acyl carrier protein （ACP） and lactate dehydrogenase （LDH）, and the contents of brain natriuretic party（BNP）, TNF-α and total glycogen were measured in cell culture fluid. RESULTS： Compared with normal control group, T-ATPase and Ca2＋-Mg2＋-ATPase activities were decreased significantly in model group; meanwhile, Na＋-K＋-ATPase activity was increased signifi- cantly, and ACP, LDH activities and BNP content in cell culture fluid were increased significantly （P〈0.05）. Compared with mod- el group, the activities of T-ATPase in treatment groups were increased significantly, while the activity of LDH in cell culture fluid was decreased significantly; when the volume ratio of ginsenoside Rgl to aconitine was 2 ： 1, protective effect was the strongest; the activities of Na＋-K＋-ATPase in aconitine group and compatibility groups were all decreased significantly, with statistical signifi- cance （P〈0.05）. The activities of ACP and BNP in cell culture fluid were all decreased in treatment groups, but the content of to- tal glycogen in cells and the TNF-α content of in cell culture fluid had no change （P〉0.05）. CONCLUSIONS： Compatibility of ginsenosides Rg1 and aconitine can impro