中文摘要：

目的：研究川芎嗪对白介素-1β（IL-1β）诱导退变的终板软骨细胞的影响。方法：体外分离培养大鼠椎间盘终板软骨细胞,并用IL-1β和川芎嗪处理,进行细胞增殖、细胞免疫化学染色和RT-PCR检测。结果：川芎嗪（6.25-25.00μmol/L）逆转IL-1β对大鼠终板软骨细胞增殖的抑制影响,抑制IL-1β诱导的软骨细胞IL-1β、肿瘤坏死因子-α（TNF-α）、环氧化酶-2（COX-2）和诱导型一氧化氮合酶（i NOS m RNA）过量表达,逆转IL-1β对于Col2-1、Aggrecan、MMP-13和ADAMTS-5 m RNA表达的影响,增加IL-1β诱导后Ⅱ型胶原的蛋白表达。结论：本研究证实川芎嗪可以抑制IL-1β诱导后软骨细胞的退变表现。

英文摘要：

Objective： To observe the protective effect of ligustrazine on degenerated endplate chondrocytes induced by interleukin-1β （IL-1β ）. Methods： Rat endplate cbondrocytes were isolated and cultured with IL-1β （10ng/mL） and ligustrazine （0-25 μmol/L） in vitro. The tests of cell proliferation, cell immunochemical staining and RT-PCR were processed. Results： Ligustrazine （6.25-25.00μm/L） could reverse the proliferation inhibition influence of rat endplate cartilage cell caused by IL-113, inhibit over expression of IL-1β, TNF-α, COX-2 and iNOS mRNA induced by IL-1 β, reverse the influence on expression of Co12-1, Aggrecan, MMP-13 and ADAMTS-5 mRNA caused by IL-1 β, and increase typeⅡ collagen protein expression after abduction of IL- 1 β. Conclusion： Ligustrazine could inhibit the degeneration performance of chondrocyte after abduction of IL- 1β.