中文摘要：

目的探讨ARK5和基质金属蛋白酶2（MMP-2）在小细胞肺癌（SCLC）组织中的表达及其临床意义。方法采用免疫组化方法检测106例SCLC癌组织（SCLC组）及40例正常肺组织（对照组）中ARK5和MMP-2的表达，分析ARK5、MMP-2的表达与患者病情分期及预后的关系。结果SCLC组ARK5和MMP-2阳性率分别为67．9％（72／106）和65．1％（69／106），对照组中分别为5．0％（2／40）和7．5％（3／40），差异均有统计学意义（χ2值分别为46．004和38．540，P〈0．05）。ARK5和MMP～2的阳性率与肿瘤直径、分化程度、浸润深度、淋巴结转移及肿瘤分期有关（P〈0．05）。Spearman秩相关分析显示，SCLC癌组织中ARK5与MMP-2呈正相关（r=0．878．P〈0．001）。Kaplan-Meier生存曲线显示，ARK5阳性组5年生存率为12．9％，阴性组为59．6％，差异有统计学意义（χ2=30．077，P〈0．001）；MMP-2阳性组5年生存率为16．3％，阴性组为62．3％，差异有统计学意义（χ2=24．405，P〈0．001）。Cox多因素分析显示，ARK5、分化程度、肿瘤分期对SCLC患者顸后的影响有统计学意义（P〈0．05）。结论ARK5和MMP-2的表达与SCLC的分化程度、浸润深度、淋巴结转移和肿瘤分期密切相关，它们都对SCLC患者的生存率产生重大影响，同时ARK5是SCLC患者预后的独立危险因素。

英文摘要：

Objective To investigate the expression of ARK5 and MMP- 2 in small cell lung cancer （SCLC） and its clinical significance. Methods Immunohistochemistry method was used to detect the expressions of ARK5 and MMP - 2 in 106 cases of SCLC tissues and 40 cases of normal lung tissues. The expressions of ARK5 and MMP - 2 and its relationship with pa- tients＇ condition and prognosis were analyzed. Results The positive rate of ARK5 and MMP - 2 was 67. 9% （72/106） and 65.1% （69/106） respectively in SCLC group and 5.0% （2/40） and 7.5% （3/40） respectively in control group, and the difference was statistically significant （X2 were 46. 004 and 38. 540, P 〈 0.05 ） . The positive rate of ARK5 and MMP - 2 was correlated with tumor size, differentiation degree, invasion depth, lymph node metastasis and tumor stage （P 〈 0.05 ）. Spearman correlation analysis showed that there was positive correlation between the expressions of ARK5 and MMP - 2 （ r = 0. 878, P 〈 0. 001 ） . Kaplan -Meier survival analysis showed that the five -year survival rate in patients with positive ARK5 ex- pressions was 12. 9% , significantly lower than that of patients with negative expression （χ2 = 30. 077, P 〈 0. 001 ）, while the five -year survival rate in patients with positive MMP -2 expression was 16. 3%, significantly lower than that of patients with negative expression 62. 3% （χ2 = 24. 405, P 〈 0. 001 ） . Cox multi - factor analysis showed that ARK5, differentiation degree, tumor stage were independent prognostic factors for SCLC （ P 〈 0. 05） . Conclusion The expressions of ARK5 and MMP - 2 are closely related to differentiation degree, invasion depth, and lymph node metastasis and tumor stage in SCLC patients. ARK5 and MMP -2 can greatly influence patients＇ survival rate, and meanwhile, ARK5 is an independent risk factor for the prognosis of SCLC patients.