中文摘要：

目的：探讨平阳霉素（PYM）联合透明质酸钠（HA）对体外培养的人淋巴管畸形内皮细胞（HLMECs）的作用。方法对新鲜婴幼儿淋巴管畸形组织进行HLMECs原代培养。利用淋巴管内皮特异性标志物LYVE1和PROX1染色，将不同浓度（1、10、100、500μg／mL）的PYM和（50、100、300、500μg／mL）HA分别作用于HLMECs 12、24、48 h，观察对HLMECs增殖的影响并筛选其最适有效浓度。然后用该有效浓度的PYM和HA共同作用于HLMECs，在第24小时时，MTT法检测细胞生长情况，TUNEL法检测细胞凋亡情况。结果①淋巴管畸形组织和 HLMECs表达LYVE1和PROX1；②PYM的最适浓度为10μg／mL，HA的最适浓度为100μg／mL；③PYM联合HA在抑制细胞增殖和促进细胞凋亡方面比单纯的PYM作用强。结论 PYM联合HA有望在临床上用于治疗淋巴管畸形。

英文摘要：

Objective To explore the effects of pingyangmycin （PYM）combined with sodium hyaluronate （HA）on human lymphatic malformations endothelial cells （HLMECs）in vitro.Methods Primary HLMECs were isolated from a HLMs tissue and cultured with endothelial cell medium （ECM）.By lymphatic endothelial specific marker LYVE1 and PROX1 staining,the HLMs specimen and HLMECs were identified.The proliferation effects of HLMECs treated with gradient densities of PYM （1,10,100,500 μg/mL）or HA （50,100,300,500 μg/mL）for 12,24 and 48 hours,were compared with MTT assay,and the optimal concentration of PYM and HA were selected.After that,the HLMECs were treated with PYM and HA of the optimal concentration.After 24 hours,the cell proliferation and apop-tosis were evaluated with MTT assay and TUNEL respectively.Results ① Both LYVE1 and PROX1 were expressed in the HLMs tissue and HLMECs.② The optimal concentration of PYM and HA was 10 μg/mL and 100 μg/mL respectively.③ The combination of PYM and HA was more effective than PYM alone on inhibiting HLMECs growth and promoting HLMECs apoptosis.Conclusion The combination of PYM and HA is effective for HLMECs,which can be an alternative in the clinical treatment of lymphatic malformations.