中文摘要：

我们开发了韦斯特尼罗河病毒(WNV ) 的 Gaussia 酶(Gluc ) 记者 replicon 并且使用了它确定病毒的翻译和 RNA 复制。Gluc replicon 的优点是 Gaussia 酶与 Gluc replicon RNA 从房间 transfected 被分泌进文化媒介，并且媒介能为酶活动直接是 assayed。用一个已知的 Flavivirus 禁止者(NITD008 ) ，我们证明 Gluc-WNV replicon 能被用于抗病毒的屏蔽。Gluc-WNV-Rep 将为在抗病毒的药发展节目的研究，以及为学习 WNV 的病毒的复制和致病是有用的。

英文摘要：

We developed a Gaussia luciferase （Gluc） reporter replicon of West Nile virus （WNV） and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor （NITD008）, we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.