目的研究反柄紫芝Ganoderma cochlear子实体的化学成分。方法利用硅胶和凝胶柱色谱及半制备HPLC进行分离纯化,采用多种谱学技术(1D、2D NMR)并根据理化常数鉴定结构。结果分离并鉴定了5个化合物,分别为拱状灵芝素G(1)、拱状灵芝素H(2)、麦角甾醇(3)、5α,8α-环二氧-6,22-麦角甾二烯-3β-醇(4)和(22E,24R)-7,22-麦角甾二烯-3β-醇(5)。结论化合物1和2为新化合物,其余化合物均为首次从灵芝属真菌中分离得到。
Objective To study the chemical constituents from the sporophore of Ganoderma cochlear.Methods The methanol extracts of G.cochlear were treated by repeated silica gel,Sephadex LH-20 column chromatography and semi-preparative HPLC.Structures were identified by physicochemical data and 1D and 2D NMR technology.Results From the sporophore of G.cochlear,two new 3,4-seco-trinorlanostane triterpenoids,named fornicatin G(1) and fornicatin H(2),together with three known ergostane sterols,named ergosterol(3),5α,8α-epi-dioxyergosta-6,22-dien-3β-ol(4),and(22E,24R)-ergosta-7,22-dien-3β-ol(5) were isolated.Conclusion Compounds 1 and 2 are new and other compounds are isolated from this fungus of Ganoderma Karst.for the first time.