中文摘要：

研究了不同浓度下（0（对照）、0.1、1、10、100μg·L-1）壬基酚（NP）长期暴露对斑马鱼（Brachydanio rerio）雄鱼第二性征、精子活力的影响.结果表明,NP暴露对斑马鱼第二性征的影响显著,可导致雄性个体出现泄殖乳突、腹部膨大等典型雌性化特征.100μg·L-1NP暴露导致76.9%的雄鱼出现泄殖乳突.NP暴露对斑马鱼精子激活率的影响显著,随NP暴露浓度升高精子激活率下降,呈现负相关的剂量-效应关系.NP暴露对斑马鱼精子寿命和精子剧烈运动时间均有显著影响.低剂量（0.1μg·L-1）和高剂量（100μg·L-1）NP暴露下精子寿命及其剧烈运动时间显著缩短,而中等剂量（10μg·L-1）NP对上述指标影响不显著（p〉0.05）.斑马鱼第二性征、精子活力可作为指示水体环境雌激素毒理学效应的敏感指标.

英文摘要：

The present work examined the effects of long-term exposed to different nonylphenol（NP）concentrations（0（control）,0.1,1,10,100μg·L-1） on secondary sexual characteristics and sperm motility of male zebrafish（Brachydanio rerio）.Results showed that male secondary sexual characteristics were significantly affected by NP exposure,showing visible urogenital papillae and bulgy abdomen,which belong to the female.76.9% males were feminised with development of urogenital papillae at 100μg·L-1 NP.Sperm activation rate showed a negative correlation with NP concentration.NP exposure also showed marked influence on acute movement time and longevity of the zebrafish sperm.Both the above two parameters were clearly shorter at low-dose（0.1μg·L-1） and high-dose（100μg·L-1） treatment comparing with the control.However,it was similar with the control at medium-dose（10μg·L-1）.The results suggested that the secondary sexual characteristics and sperm motility of male zebrafish might be used as the sensitive indexes of aquatic environmental endocrine disrupting chemicals.