中文摘要：

目的 探讨循环牵张应力对体外培养的人骨关节炎患者软骨细胞增殖的影响。方法 退变软骨来源于1例66岁骨关节炎患者的手术取材，病理学诊断评估其退变程度。无菌条件下酶消化法原代培养退变软骨细胞，并用甲苯胺蓝、番红 O 和Ⅱ型胶原免疫荧光染色进行鉴定。将第 3 代细胞接种于硅橡胶膜载体上，用 Electro Force3200力学试验仪搭载的BioDynamic生物反应仓对其施加0、5%、10%和15%的应变作用3 h，受力完成后继续培养24 h，用流式细胞仪测定不同力学环境下细胞的增殖活性。结果 随着牵张应力的增加，S期细胞百分比和增殖指数（PI）呈缓慢上升趋势，10%时达到峰值，之后开始下降。结论 体外培养的人骨关节炎软骨细胞的增殖活性与受力大小有关，且存在细胞增殖的最适应力条件；超过此应力范围其增殖活性将会降低。

英文摘要：

Objective To explore the effects of cyclic tensile strain on the proliferation of cultured human osteoar- thritis chondrocytes. Methods The degenerated human cartilage came from a 66-year-old patient with osteoarthritis. The degree of degeneration was assessed by pathological diagnosis. Under the sterile condition, degenerated chondrocytes were cultured by enzymatic digestion. The toluidine blue staining, safranin O staining, and immunofluorescence staining of colla- gen 1[ were used to identify the euhured cells. The third generation cells were seeded on the silicone rubber membrane carri- er, using the EF3200 mechanical tester equipped with BioDynamic bioreactor system, imposed a frequency of 0.25 Hz, stain 0, 5%, 10% and 15% load for 3 h. The proliferation activity was detected by flow cytometry. Results The proliferative in- dex （PI） increased with the magnitude value of cyclic tensile strain. The most significant increase of proliferation index was found in 10% group. Conclusion The proliferation of human osteoarthritis chondrocytes increases under some stress.