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Over-Expression of ScMnSOD,a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis
  • ISSN号:0476-0301
  • 期刊名称:《北京师范大学学报:自然科学版》
  • 时间:0
  • 分类:Q943.2[生物学—植物学] TS252.1[轻工技术与工程—农产品加工及贮藏工程;轻工技术与工程—食品科学与工程]
  • 作者机构:[1]Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R.China, [2]College of Life Sciences, Beijing Normal University, Beijing 100875, P.R.China, [3]COE For Neuroscience, Departments of Anesthesiology, Biomedical Sciences, Texas Tech University Health Sciences Center, Texas 79905, USA
  • 相关基金:supported by the National Natural Sciences Foundation of China (30970286, 31270362 and 31070289)
中文摘要:

Jojoba(Simmondsia chinensis) is mainly distributed in desert,and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive.In this paper,we cloned and characterized a SOD gene from jojoba named as ScMnSOD,and introduced into Arabidopsis to investigate its functions of responding to drought stress.The transgenic Arabidopsis showed an improvement in drought tolerance.Moreover,under a water deficit condition,the accumulation of reactive oxygen species(ROS) was remarkably decreased in the transgenic lines compared to the WT.Furthermore,the ScMnSOD promoter was cloned to the 5′-upstream of GUS coding region in a binary vector,and introduced into Arabidopsis.And results showed that ScMnSOD expression can be induced by drought,salt,ABA,and low temperature.In conclusion,ScMnSOD plays an important role in drought tolerance which is,at least partially,attributed to its role in ROS detoxification.

英文摘要:

Jojoba(Simmondsia chinensis) is mainly distributed in desert,and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive.In this paper,we cloned and characterized a SOD gene from jojoba named as ScMnSOD,and introduced into Arabidopsis to investigate its functions of responding to drought stress.The transgenic Arabidopsis showed an improvement in drought tolerance.Moreover,under a water deficit condition,the accumulation of reactive oxygen species(ROS) was remarkably decreased in the transgenic lines compared to the WT.Furthermore,the ScMnSOD promoter was cloned to the 5′-upstream of GUS coding region in a binary vector,and introduced into Arabidopsis.And results showed that ScMnSOD expression can be induced by drought,salt,ABA,and low temperature.In conclusion,ScMnSOD plays an important role in drought tolerance which is,at least partially,attributed to its role in ROS detoxification.

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期刊信息
  • 《北京师范大学学报:自然科学版》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国教育部
  • 主办单位:北京师范大学
  • 主编:刘文彪
  • 地址:北京新外大街19号
  • 邮编:100875
  • 邮箱:JBNUNS@bnu.EDU.CN
  • 电话:
  • 国际标准刊号:ISSN:0476-0301
  • 国内统一刊号:ISSN:11-1991/N
  • 邮发代号:82-406
  • 获奖情况:
  • 1997年全国第二届科技期刊评比一等奖,1999年教育部优秀科技期刊二等奖,1999年首届国家期刊奖,中国期刊方阵“双高”期刊
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),美国数学评论(网络版),德国数学文摘,英国科学文摘数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:10672