中文摘要：

以黄瓜叶片中提取的总RNA为模板,经PCR扩增得到长度为1 275bp的基因片断,经过酶切测序之后,将目的基因连接到表达载体,构建成重组质粒。再利用液氮冻融法将表达载体转化农杆菌感受态。

英文摘要：

STS gene of 1 275bp length from Cucumis sativus were cloned by RT-PCR,and were connected with cloned-vector.After sequencing and comparing with the gene showed on Genbank,absolutely accurate gene se quence which was amplied with two peculiar primier and primestar enzyme were inserted into expression vector。 STS recombined plasmid were successfully constructed,then were transformed to Agrobacterium.